冷冻保存后人精子中谷胱甘肽过氧化物酶和谷胱甘肽还原酶基因表达的比较分析
Comparative Analysis of the Expression of Glutathione Peroxidase and Glutathione Reductase Genes in Human Sperm after Cryopreservation.
作者信息
Makarova N P, Romanov A Yu, Dolgushina N V, Parker M M, Krasnyi A M
机构信息
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2018 May;165(1):166-170. doi: 10.1007/s10517-018-4121-y. Epub 2018 May 24.
We analyzed the effect of cryopreservation on the expression of glutathione peroxidase (GPX1) and glutathione reductase (GSR) genes in human sperm cells (15 sperm samples from fertile donors and 10 samples from infertile patients). The relative expression of GPX1 and GSR genes was determined by real-time PCR. The rate of post-thaw recovery was 2.1 times higher in the group of fertile donors. A significant increase in the expression of GPX1, but not GSR, was observed in sperm samples from infertile patients, while in patients with infertility, GPX1 expression significantly decreased after cryopreservation/thawing, in samples from fertile donors after the same procedure it increased to the level observed in the sperm samples from infertile patients. A positive correlation was revealed between GPX1 expression and sperm cryotolerance.
我们分析了冷冻保存对人类精子细胞中谷胱甘肽过氧化物酶（GPX1）和谷胱甘肽还原酶（GSR）基因表达的影响（来自生育能力正常供体的15份精子样本和来自不育患者的10份样本）。通过实时PCR测定GPX1和GSR基因的相对表达。生育能力正常供体组的解冻后复苏率高出2.1倍。在不育患者的精子样本中观察到GPX1表达显著增加，但GSR未增加，而在不育患者中，冷冻保存/解冻后GPX1表达显著降低，在生育能力正常供体的样本中经过相同程序后其增加到不育患者精子样本中观察到的水平。GPX1表达与精子冷冻耐受性之间存在正相关。
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