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利用离子液体预处理甘蔗渣后的毕赤酵母(Schizosaccharomyces pombe)高效转化为乙醇。

Schizosaccharomyces pombe as an Efficient Yeast to Convert Sugarcane Bagasse Pretreated with Ionic Liquids in Ethanol.

机构信息

University of Caxias do Sul - Institute of Biotechnology - Enzyme and Biomass Laboratory, 1130, Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil.

出版信息

Appl Biochem Biotechnol. 2018 Dec;186(4):960-971. doi: 10.1007/s12010-018-2788-1. Epub 2018 May 25.

Abstract

Pretreatment of lignocellulosic biomass with ionic liquids (ILs) has been extensively studied, being regarded as one of the most promising methods for obtaining fermentable sugars. In this research, it was investigated the production of ethanol from sugars released from sugarcane bagasse pretreated with the ionic liquids [Cmim][OAc] and [Cmim][OAc], hydrolysed with Penicillium echinulatum enzymes and using Saccharomyces cerevisiae and Schizosaccharomyces pombe. Yields of about 43 and 56% of ethanol were observed for S. cerevisiae and biomass pretreated with [Cmim][OAc] and [Cmim][OAc], respectively. S. pombe yielded 52 and 78% ethanol for [Cmim][OAc] and [Cmim][OAc], respectively. These results indicate that S. pombe showed best performance for alcoholic fermentation from sugars released from pretreated biomass by ILs.

摘要

预处理木质纤维素生物质与离子液体(ILs)已被广泛研究,被认为是获得可发酵糖的最有前途的方法之一。在这项研究中,研究了从用离子液体[Cmim][OAc]和[Cmim][OAc]预处理的甘蔗渣中释放的糖生产乙醇,用青霉酶水解,然后用酿酒酵母和粟酒裂殖酵母发酵。酿酒酵母和用[Cmim][OAc]预处理的生物质分别得到约 43%和 56%的乙醇产率。粟酒裂殖酵母用[Cmim][OAc]和[Cmim][OAc]分别得到 52%和 78%的乙醇。这些结果表明,粟酒裂殖酵母在从 ILs 预处理生物质中释放的糖发酵生产乙醇方面表现出最佳性能。

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