State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, Zhejiang, China.
School of Medicine and School of Biomedical Sciences, Huaqiao University, Xiamen, Fujian, China.
Microbiologyopen. 2019 Feb;8(2):e00634. doi: 10.1002/mbo3.634. Epub 2018 May 23.
Myroides odoratimimus is an important nosocomial pathogen. Management of M. odoratimimus infection is difficult owing to the multidrug resistance and the unknown pathogenesis mechanisms. Based on our previous genomic sequencing data of M. odoratimimus PR63039 (isolated from a patient with the urinary tract infection), in this study, we further performed comparative genomic analysis for 10 selected Myroides strains. Our results showed that these Myroides genome contexts were very similar and phylogenetically related. Various prophages were identified in the four clinical isolate genomes, which possibly contributed to the genome evolution among the Myroides strains. CRISPR elements were only detected in the two clinical (PR63039 and CCUG10230) isolates and two environmental (CCUG12700 and H1bi) strains. With more stringent cutoff parameters in CARD analysis, the four clinical M. odoratimimus contained roughly equal antibiotic resistance genes, indicating their similar antibiotic resistance profiles. The three clinical (CCUG10230, CCUG12901, CIP101113) and three environmental (CCUG12700, L41, H1bi) M. odoratimimus strains were speculated to carry the indistinguishable virulent factors (VFs), which may involve in the similar pathogenesis mechanism. Moreover, some VFs might confer to the high capacity of dissemination, attacking tissue cells and induction of autoimmune complications. Our results facilitate the research of antibiotic resistance and the development of therapeutic regimens for the M. odoratimimus infections.
恶臭微球菌是一种重要的医院病原体。由于其具有多种药物耐药性和未知的发病机制，因此对恶臭微球菌感染的管理具有一定难度。基于我们之前对恶臭微球菌 PR63039（从尿路感染患者中分离得到）的基因组测序数据，在本研究中，我们进一步对 10 株选定的恶臭微球菌菌株进行了比较基因组分析。我们的结果表明，这些恶臭微球菌的基因组结构非常相似，且具有系统发育相关性。在四个临床分离株基因组中鉴定出了各种噬菌体，这可能有助于恶臭微球菌菌株之间的基因组进化。仅在两个临床（PR63039 和 CCUG10230）和两个环境（CCUG12700 和 H1bi）分离株中检测到 CRISPR 元件。在 CARD 分析中使用更严格的截止参数时，这四个临床恶臭微球菌菌株大致含有相等数量的抗生素耐药基因，表明它们具有相似的抗生素耐药谱。三个临床（CCUG10230、CCUG12901 和 CIP101113）和三个环境（CCUG12700、L41 和 H1bi）恶臭微球菌菌株被推测携带难以区分的毒力因子（VF），这可能涉及相似的发病机制。此外，一些 VF 可能赋予其较强的传播能力，攻击组织细胞并诱导自身免疫并发症。我们的研究结果有助于研究恶臭微球菌的抗生素耐药性，并为恶臭微球菌感染的治疗方案的制定提供了一定的依据。
