The State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China; Department of Epidemiology, the First Affiliated Hospital of Shandong First Medical University, Jinan 250062, China; Shandong Medicinal Biotechnology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China.
School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, China.
Sci Total Environ. 2020 Jun 25;723:137970. doi: 10.1016/j.scitotenv.2020.137970. Epub 2020 Mar 16.
Strains of the environmental bacterium Myroides odoratimimus can cause human infections. However, treating M. odoratimimus infections can be difficult because of multidrug resistance in this organism. In this study, we isolated strain M. odoratimimus G13 from pastureland in Tibet, China. The minimum inhibitory concentration analysis suggested that strain G13 has resistance to multiple antibiotics, with an MIC for tetracycline of 168 mg/L. Whole-genome sequencing and bioinformatic analysis revealed that the genome of G13 was rich in virulence factor-encoding genes and antibiotic resistance genes (ARGs). The mobilizable genomic island MGI1313 was also identified and characterized, and six resistance genes related to four types of antibiotics were annotated in MGI1313. Conjugation assays indicated that MGI1313 could be transferred from G13 to Escherichia coli 25DN by horizontal gene transfer, resulting in multidrug-resistant E. coli conjugants. In conclusion, multidrug-resistant M. odoratimimus G13 and the mobility of MGI1313 raise the risk of difficult-to-treat bacterial infections and should be under close surveillance.
环境细菌粘质沙雷氏菌的菌株可引起人类感染。然而，由于该生物体的多药耐药性，治疗粘质沙雷氏菌感染可能很困难。在这项研究中，我们从中国西藏的牧场中分离出了粘质沙雷氏菌 G13 菌株。最低抑菌浓度分析表明，菌株 G13 对多种抗生素具有耐药性，其四环素的 MIC 为 168mg/L。全基因组测序和生物信息学分析表明，G13 的基因组富含毒力因子编码基因和抗生素耐药基因 (ARGs)。还鉴定和表征了可移动基因组岛 MGI1313，并在 MGI1313 中注释了与四种类型抗生素相关的六个耐药基因。接合实验表明，MGI1313 可通过水平基因转移从 G13 转移到大肠杆菌 25DN，导致多药耐药性大肠杆菌接合子。总之，耐多药粘质沙雷氏菌 G13 和 MGI1313 的可移动性增加了难以治疗的细菌感染的风险，应密切监测。
