Zhang Yanqi, Zhang Limin, Yin Guoyan, Ma Wenjuan, Gao Feng
College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, China.
Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin, China.
J Biophotonics. 2018 Oct;11(10):e201800041. doi: 10.1002/jbio.201800041. Epub 2018 Jun 19.
Fluorescence pharmacokinetic rates in tissues can provide additional specific and quantitative physiological and pathological information for evaluating organ function. This modality requires a highly sensitive diffuse fluorescence tomography (DFT) working in dynamic way to finally extract the pharmacokinetic rates from the measured pharmacokinetics-associated temporally varying boundary intensity, normally with the support of a priori anatomy. This paper is devoted to study pharmacokinetics of indocyanine green (ICG) in mouse liver based on synergistic dynamic-DFT and X-ray computer tomography (XCT): A highly sensitive dynamic DFT system of CT-scanning mode working with parallel 4 photomultiplier-tube photon-counting channels generates informative and instantaneous sampling datasets; An XCT system provides priori information of the target localization for improvement of the reconstruction quality; An analysis procedure extracts the pharmacokinetic rates from the reconstructed ICG concentration-time curves, using the Gauss-Newton scheme for fitting to a 2-compartment model. The uptake and excretion rates of ICG which were obtained in livers of 10 healthy mice in the in vivo experiments can be used to quantitatively evaluate liver function. The results can validate the effectiveness of both the imaging measurements system and pharmacokinetic analysis method.
组织中的荧光药代动力学速率可为评估器官功能提供额外的特定和定量生理及病理信息。这种方法需要一个以动态方式工作的高灵敏度扩散荧光断层扫描（DFT），以便最终从测量的与药代动力学相关的随时间变化的边界强度中提取药代动力学速率，通常需要先验解剖结构的支持。本文致力于基于协同动态DFT和X射线计算机断层扫描（XCT）研究吲哚菁绿（ICG）在小鼠肝脏中的药代动力学：一个具有平行4个光电倍增管光子计数通道的CT扫描模式的高灵敏度动态DFT系统生成信息丰富且即时的采样数据集；一个XCT系统提供目标定位的先验信息以提高重建质量；一个分析程序使用高斯 - 牛顿法拟合二室模型，从重建的ICG浓度 - 时间曲线中提取药代动力学速率。在体内实验中从10只健康小鼠肝脏获得的ICG摄取和排泄速率可用于定量评估肝功能。结果可以验证成像测量系统和药代动力学分析方法的有效性。
