Shulman S
Sperm Antibody Laboratory, Fertility Antibody Diagnostics, Inc., New York, New York 10025.
In Vivo. 1988 Jan-Feb;2(1):57-60.
Both men and women make antibodies against human sperm. For a complete diagnostic evaluation of an infertile couple, we must test the two serum samples, plus her cervical mucus and his sperm cells. The antibody factor occurs in 9% of men and in 12-15% of women from infertile couples, and at various titers. The standard, or classical, methods for antibody detection in serum include several agglutination methods: Gelatin Agglutination Test (GAT), Tumbe-Slide Agglutination Test (TSAT), Tray Agglutination Test (TAT), and Capillary Tube Agglutination Test (CTAT), plus the immobilization (Isojima). There are also newer methods; passive hemagglutination, radiolabel-antiglobulin, ATR-release cytotoxicity, hemadsorption, indirect immunobead, and ELISA. The genital secretions must also be studied. Sperm cells are judged to be antibody-coated by use of the Mixed Anti-Globulin Reaction (MAR) test or the Immunobead Test (IBT). Cervical mucus is dissolved with bromelin and tested.
男性和女性都会产生抗人类精子的抗体。对于一对不孕夫妇进行全面的诊断评估时，我们必须检测双方的血清样本，以及她的宫颈黏液和他的精子细胞。抗体因素在不孕夫妇中，男性出现比例为9%，女性为12% - 15%，且滴度各异。血清中抗体检测的标准或经典方法包括几种凝集方法：明胶凝集试验（GAT）、 Tumbe - 玻片凝集试验（TSAT）、托盘凝集试验（TAT）和毛细管凝集试验（CTAT），以及免疫固定法（稻岛法）。还有一些更新的方法：被动血凝试验、放射性标记抗球蛋白试验、ATR释放细胞毒性试验、血细胞吸附试验、间接免疫珠试验和酶联免疫吸附测定（ELISA）。生殖分泌物也必须进行研究。通过混合抗球蛋白反应（MAR）试验或免疫珠试验（IBT）来判断精子细胞是否被抗体包被。宫颈黏液用菠萝蛋白酶溶解后进行检测。
