Wang S B, Zhang H W, Xi Z L, Huang J J, Nie J, Zhou B, Chen S M, Tao Z Z
Department of Otolaryngology-Head and Neck Surgery,Yichang Yiling Hospital,Yichang,443100,China.
Department of Otolaryngology-Head and Neck Surgery,Renmin Hospital of Wuhan University.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2016 Sep 5;30(17):1376-1380. doi: 10.13201/j.issn.1001-1781.2016.17.009.
To explore the effect of lipopolysaccharide(LPS) on the formation of nasal polyps in mice and its mechanism.Five or ten μg of LPS were dropped into the nasal cavities of six to eight-week C57BL/6J mice in order to establish animal models with nasal polyps.Buried food pellets experiment was used to assess olfactory function of mice.Hematoxylin-eosin staining,olfactory marker protein(OMP),and myeloperoxidase(MPO) for neutrophil immunohistochemistry were used to observe histopathological changes in the nasal mucosa.The expression levels of cytokines,such as interferon(IFN)-γ,tumor necrosis factor(TNF)-α,IL-6 in the nasal lavage fluid,were detected by ELISA.Compared to the control group,mice in the LPS groups showed significant nasal polyp formation,olfactory dysfunction,more MPO⁺ cells while less OMP⁺ cells in the nasal mucosa,as well as increased significantly levels of IFN-γ,TNF-α,and IL-6 in the nasal lavage fluids(all <0.05).LPS from Gram negative bacteria alone can induce the formation of nasal polyps in mice,characterized by more neutrophils,olfactory dysfunction,and increasing Th1 cytokine.Therefore,this model is commensurate with the characteristics of primary nasal polyps found in China and other major Asian.The current study suggests that LPS may be one of the important pathogenesis of gram negative bacteria in the nasal polyps.
探讨脂多糖（LPS）对小鼠鼻息肉形成的影响及其机制。将5或10μg LPS滴入6至8周龄C57BL/6J小鼠的鼻腔，以建立鼻息肉动物模型。采用埋食颗粒实验评估小鼠的嗅觉功能。用苏木精-伊红染色、嗅觉标记蛋白（OMP）以及中性粒细胞免疫组化的髓过氧化物酶（MPO）观察鼻黏膜的组织病理学变化。通过ELISA检测鼻腔灌洗液中细胞因子如干扰素（IFN）-γ、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6的表达水平。与对照组相比，LPS组小鼠出现明显的鼻息肉形成、嗅觉功能障碍，鼻黏膜中MPO⁺细胞增多而OMP⁺细胞减少，鼻腔灌洗液中IFN-γ、TNF-α和IL-6水平显著升高（均P<0.05）。单独的革兰氏阴性菌LPS可诱导小鼠鼻息肉形成，其特征为中性粒细胞增多、嗅觉功能障碍以及Th1细胞因子增加。因此，该模型与在中国及其他主要亚洲国家发现的原发性鼻息肉特征相符。当前研究表明，LPS可能是革兰氏阴性菌在鼻息肉发病中的重要因素之一。
