Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing 100191, China.
Asian J Androl. 2018 Sep-Oct;20(5):454-458. doi: 10.4103/aja.aja_27_18.
In the present study, we evaluated the impact of sperm origins and concentration on the clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles. A total of 1201 ICSI cycles were retrospectively analyzed for male azoospermia or oligozoospermia between January 2015 and December 2015 in the Peking University Third Hospital. Patients were divided into three groups (Group 1 vs Group 2/3; surgically extracted sperm vs ejaculated sperms): Group 1 included 343 ICSI cycles and Group 2 analyzed 388 cycles on semen with sperm concentration <5 × 10 ml (severe oligozoospermia group). Group 3 included 470 cycles with sperm concentration between 5 × 10 ml and 15 × 10 ml (mild oligozoospermia group). Fertilization rates, clinical pregnancy rates, and live birth rates were analyzed and compared among groups of different semen origins and concentrations on the oocyte retrieval day. Group 2 showed a lower fertilization rate than Group 3 (62.9% ± 21.6% vs 66.8% ± 22.1%,P< 0.05). There were no statistically significant differences in clinical pregnancy rate per transfer (51.3%, 46.7%, and 50.0%, respectively), live birth rate per transfer (44.4%, 40.9%, and 41.4%, respectively), accumulative live birth rate (58.3%, 51.0%, and 52.1%, respectively), twin birth rate (18.4%, 10.6%, and 12.6%, respectively), and birth defects rate (0, 0.3%, and 0.2%, respectively) among three groups. The results of this study indicated that sperm origins and concentration do not impact the clinical outcomes in ICSI cycles.
在本研究中，我们评估了精子来源和浓度对卵胞浆内单精子注射（ICSI）周期临床结局的影响。回顾性分析了 2015 年 1 月至 2015 年 12 月北京大学第三医院因男性无精子症或严重少精子症行 ICSI 治疗的 1201 个周期。根据精子来源和浓度将患者分为三组（组 1 与组 2/3；手术提取精子与射出精子）：组 1 包括 343 个 ICSI 周期，组 2 分析了 388 个精子浓度＜5×10/ml（严重少精子症组）的周期，组 3 包括 470 个精子浓度为 5×10/ml-15×10/ml（轻度少精子症组）的周期。比较了不同精子来源和浓度组在取卵日的受精率、临床妊娠率和活产率。组 2 的受精率明显低于组 3（62.9%±21.6% vs 66.8%±22.1%，P＜0.05）。每组间的临床妊娠率移植周期（51.3%、46.7%和 50.0%）、活产率移植周期（44.4%、40.9%和 41.4%）、累积活产率（58.3%、51.0%和 52.1%）、双胎妊娠率（18.4%、10.6%和 12.6%）和出生缺陷率（0、0.3%和 0.2%）均无统计学差异。本研究结果表明，精子来源和浓度并不影响 ICSI 周期的临床结局。
