Research Laboratoryé of the Undergraduate and Postgraduate Dentistry Program, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, Mexico; Endodontic Specialization Program, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, México.
Endodontic Specialization Program, Facultad de Medicina, Universidad Autónoma de Querétaro, Santiago de Querétaro, México.
J Glob Antimicrob Resist. 2018 Dec;15:20-24. doi: 10.1016/j.jgar.2018.05.011. Epub 2018 May 22.
The aim of this study was to identify the prevalence of genes encoding resistance to three groups of antimicrobial agents in root canal samples from primary infection or post-treatment disease in Mexico.
A total of 64 subjects requiring root canal treatment because of primary infection or post-treatment disease were enrolled in this cross-sectional analytical study. Root canal samples were obtained and DNA was isolated. Specific primers for six antimicrobial resistance genes (ARGs) and seven bacterial taxa (five genera and two species) were used. Student's t-test, χ test and Fisher's exact test were applied where appropriate to detect statistical differences.
The bla, ermC and tetM genes were found more frequently in the post-treatment disease group compared with the primary infection group. The occurrence of assessed bacteria was similar in both groups, except for Enterococcus spp. and Porphyromonas endodontalis, which were found at a significantly higher frequency in the post-treatment disease group. It was observed that the post-treatment disease group harboured more ARGs. The most frequent ARG was tetW, whereas tetQ and cfxA were not detected in any case. With respect to bacterial taxa, Fusobacterium spp. was present in 100% of samples, whereas Porphyromonas gingivalis was not observed in any of the samples.
At least one ARG was detected in all cases; moreover, 32.8% of samples were positive for four ARGs, 54.7% for three ARGs, 9.4% for two ARGs and only 3.1% for one ARG. This indicates a high prevalence and diversity of ARGs in these root canal samples.
本研究旨在确定墨西哥初次感染或治疗后根管样本中编码对三组抗菌药物耐药的基因的流行率。
本横断面分析性研究共纳入 64 例因初次感染或治疗后疾病需行根管治疗的患者。采集根管样本并提取 DNA。使用针对 6 种抗菌药物耐药基因（ARGs）和 7 种细菌分类群（5 个属和 2 个种）的特异性引物。应用学生 t 检验、χ 检验和 Fisher 确切检验来检测统计学差异。
与初次感染组相比，治疗后疾病组中 bla、ermC 和 tetM 基因的检出率更高。两组的评估细菌发生情况相似，除治疗后疾病组中 Enterococcus spp. 和 Porphyromonas endodontalis 的检出率显著更高外。观察到治疗后疾病组携带更多的 ARGs。最常见的 ARG 是 tetW，而 tetQ 和 cfxA 在任何情况下均未检出。关于细菌分类群，Fusobacterium spp. 在所有样本中均存在，而 Porphyromonas gingivalis 在任何样本中均未检出。
所有病例均检出至少 1 种 ARG；此外，32.8%的样本对 4 种 ARG 呈阳性，54.7%对 3 种 ARG 呈阳性，9.4%对 2 种 ARG 呈阳性，仅有 3.1%对 1 种 ARG 呈阳性。这表明这些根管样本中 ARGs 的流行率和多样性均较高。
