Universidad Autónoma de Querétaro, Facultad de Medicina, Laboratorio de Investigación Odontológica Multidisciplinaria, Santiago de Querétaro, México.
Universidad Autónoma de Querétaro, Facultad de Medicina, Departamento de Investigación Biomédica, Santiago de Querétaro, México.
J Appl Oral Sci. 2023 Feb 3;30:e20220362. doi: 10.1590/1678-7757-2022-0362. eCollection 2023.
Antimicrobial resistance is a global public health problem. Root canal microbiota associated with apical periodontitis represents a well-known reservoir of antimicrobial resistance genes (ARGs). However, the effect of type 2 diabetes mellitus (T2DM) in this reservoir is unknown. This study aimed to establish if root canal microbiota associated with apical periodontitis in T2DM patients is an augmented reservoir by identifying the prevalence of nine common ARGs and comparing it with the prevalence in nondiabetic patients.
This cross-sectional study included two groups: A T2DM group conformed of 20 patients with at least ten years of living with T2DM and a control group of 30 nondiabetic participants. Premolar or molar teeth with pulp necrosis and apical periodontitis were included. A sample was collected from each root canal before endodontic treatment. DNA was extracted, and ARGs were identified by polymerase chain reaction.
tetW and tetM genes were the most frequent (93.3 and 91.6%, respectively), while ermA was the least frequent (8.3%) in the total population. The distribution of the ARGs was similar in both groups, but a significant difference (p<0.005) was present in ermB, ermC, cfxA, and tetQ genes, being more frequent in the T2DM group. A total of eighty percent of the T2DM patients presented a minimum of four ARGs, while 76.6% of the control group presented a maximum of three.
Root canal microbiota associated with apical periodontitis in T2DM patients carries more ARGs. Therefore, this pathological niche could be considered an augmented reservoir.
抗菌药物耐药性是一个全球性的公共卫生问题。与根尖周炎相关的根管微生物群代表了众所周知的抗菌药物耐药基因（ARGs）库。然而，2 型糖尿病（T2DM）对此库的影响尚不清楚。本研究旨在通过鉴定 9 种常见 ARGs 的流行情况，并与非糖尿病患者的流行情况进行比较，确定 T2DM 患者根尖周炎相关根管微生物群是否是一个增强的储库。
这是一项横断面研究，包括两组：一组是 T2DM 组，由 20 名至少患有 T2DM10 年的患者组成；另一组是对照组，由 30 名非糖尿病患者组成。纳入牙髓坏死和根尖周炎的前磨牙或磨牙。在牙髓治疗前，从每个根管中采集样本。提取 DNA，通过聚合酶链反应鉴定 ARGs。
tetW 和 tetM 基因最为常见（分别为 93.3%和 91.6%），而 ermA 在总人群中最为少见（8.3%）。两组 ARGs 的分布相似，但 ermB、ermC、cfxA 和 tetQ 基因在 T2DM 组中更为常见，差异具有统计学意义（p<0.005）。80%的 T2DM 患者至少携带 4 种 ARGs，而对照组中 76.6%的患者最多携带 3 种 ARGs。
与 T2DM 患者根尖周炎相关的根管微生物群携带更多的 ARGs。因此，这个病理生态位可以被认为是一个增强的储库。
