Ainsworth D M, Keith I M, Lobas J G, Farrell P M, Eicker S W
Dept. Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
Histol Histopathol. 1986 Jan;1(1):75-87.
Eight monkeys were anesthetized with ketamine hydrochloride and positive pressure ventilated with greater than 95% oxygen (tests) or room air (controls) for 24 hours. Two test monkeys and one control were treated with E. coli endotoxin (500 micrograms/kg) IV at the start of the study and after 12 hours. Histopathological changes in the lung parenchyma were evaluated using light and electron microscopy. Interstitial edema was detected as early as 24 hours after the onset of hyperoxia but there was no significant increase in the alveolar-capillary distance (blood-air barrier). Morphologic signs of oxygen toxicity further included swelling and disruption of vascular endothelium, and swelling of alveolar Type II pneumocytes. There was no difference in the number of macrophages per high power field between the four groups but significant differences were observed in the number of neuroepithelial bodies (NEBs) per cm2 and mast cells per high power field at the light microscopic level. Treatment with endotoxin did not protect against oxygen toxicity and was associated with an exacerbation of the morphological alterations in the lung parenchyma and swelling of alveolar Type I pneumocytes.
八只猴子用盐酸氯胺酮麻醉，并以高于95%的氧气（试验组）或室内空气（对照组）进行正压通气24小时。在研究开始时和12小时后，给两只试验猴和一只对照猴静脉注射大肠杆菌内毒素（500微克/千克）。使用光学显微镜和电子显微镜评估肺实质的组织病理学变化。早在高氧暴露24小时后就检测到间质性水肿，但肺泡-毛细血管距离（气血屏障）没有显著增加。氧中毒的形态学征象还包括血管内皮肿胀和破坏，以及肺泡II型上皮细胞肿胀。四组之间每高倍视野的巨噬细胞数量没有差异，但在光学显微镜水平上，每平方厘米的神经上皮小体（NEBs）数量和每高倍视野的肥大细胞数量存在显著差异。内毒素治疗不能预防氧中毒，且与肺实质形态学改变的加重以及肺泡I型上皮细胞肿胀有关。
