Pathology Department of the First Affiliated Hospital, Southwest Medical University, Taiping Street No.25, Jiangyang District, Luzhou City, Sichuan, China.
Virol J. 2018 May 25;15(1):94. doi: 10.1186/s12985-018-1003-x.
Currently, the role of human papillomavirus (HPV)-58 in southwestern China has been unexplored. Although there is some controversy, it is proposed that the viral load of HPV correlates with the severity of intraepithelial lesions.
We identified 7747 patients from south Sichuan and adjacent regions who were diagnosed with HPV between 2013 and 2017. The HR-HPV subtype distribution was analyzed and the patient's viral loads were quantified using real-time RT-PCR.
Among all 7747 patients screened for HPV genotypes, 1728 patients (22.31%) were identified as having HR-HPV subtypes. In patients without intraepithelial lesions (12.41%), HPV-52, HPV-16, and HPV-58 were the three most prevalent HR-HPV subtypes. Moreover, HPV-16, HPV-58, and HPV-33 were the most prevalent subtypes in patients with cervical intraepithelial neoplasia grade II (CINII) (42.86%) and grade III (CINIII) (59.81%), and accounted for the majority of invasive cervical cancer (ICC) (69.34%). Thus, viral loads of HPV-58, HPV-16, and HPV-33 positively correlated with the severity of cervical lesions (P < 0.001, P = 0.016, P = 0.026, respectively). Using receiver operating characteristic (ROC) curve analysis, the optimum thresholds for predicting severe intraepithelial lesions of cases (CINI, CINIII and ICC) with HPV-16, HPV-58, and HPV-33, respectively, were obtained, which were 1, 0.93, and 0.25, respectively.
In our study, we showed that HPV-16 was the most common carcinogenic HPV subtype in southwestern China followed by HPV-58 and HPV-33. Viral loads of these subtypes are associated with the severity of premalignant lesions in the cervix.
目前，人乳头瘤病毒（HPV）-58 在我国西南地区的作用尚不清楚。尽管存在一些争议，但有人提出 HPV 的病毒载量与上皮内病变的严重程度相关。
我们从 2013 年至 2017 年间在四川南部和邻近地区诊断出 HPV 的 7747 例患者中鉴定出 HPV-58。分析 HR-HPV 亚型分布，并使用实时 RT-PCR 定量患者的病毒载量。
在筛查 HPV 基因型的 7747 例患者中，有 1728 例（22.31%）被确定为 HR-HPV 亚型。在无上皮内病变的患者（12.41%）中，HPV-52、HPV-16 和 HPV-58 是三种最常见的 HR-HPV 亚型。此外，HPV-16、HPV-58 和 HPV-33 是宫颈上皮内瘤变 II 级（CINII）（42.86%）和 III 级（CINIII）（59.81%）患者中最常见的亚型，并且占浸润性宫颈癌（ICC）（69.34%）的大多数。因此，HPV-58、HPV-16 和 HPV-33 的病毒载量与宫颈病变的严重程度呈正相关（P<0.001，P=0.016，P=0.026）。使用接收者操作特征（ROC）曲线分析，分别获得 HPV-16、HPV-58 和 HPV-33 预测病例（CINI、CINIII 和 ICC）严重上皮内病变的最佳阈值，分别为 1、0.93 和 0.25。
在本研究中，我们表明 HPV-16 是我国西南地区最常见的致癌 HPV 亚型，其次是 HPV-58 和 HPV-33。这些亚型的病毒载量与宫颈癌前病变的严重程度相关。
