Chen Jiangfei, Zheng Lidan, Tian Linjie, Wang Nengzhuang, Lei Lei, Wang Yanbo, Dong Qiaoxiang, Huang Changjiang, Yang Dongren
Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou, 325035, China.
Zhejiang Gongshang University, Hangzhou, 310035, China.
Bull Environ Contam Toxicol. 2018 Jul;101(1):75-79. doi: 10.1007/s00128-018-2359-8. Epub 2018 May 25.
Perfluorooctane sulfonic acid (PFOS), as a potential endocrine disrupting chemical, is widely detected in the environment, wildlife and human. Currently few studies have documented the effects of chronic PFOS exposure on thyroid in aquatic organisms and the underlying mechanisms are largely unknown. The present study assessed the effect of chronic PFOS exposure on thyroid structure and function using zebrafish model. Zebrafish at 8 h post fertilization (hpf) were exposed to PFOS (250 µg/l) until 120 d post fertilization (dpf). Thyroid hormone (T and T) level, thyroid morphology and thyroid function related gene expression were evaluated in zebrafish at 120 dpf. Our findings demonstrated that chronic PFOS exposure altered thyroid hormone level, thyroid follicular cell structure and thyroid hormone related gene expression, suggesting the validity of zebrafish as an alternative model for PFOS chronic toxicity screening.
全氟辛烷磺酸（PFOS）作为一种潜在的内分泌干扰化学物质，在环境、野生动物和人类中广泛被检测到。目前，很少有研究记录慢性PFOS暴露对水生生物甲状腺的影响，其潜在机制在很大程度上尚不清楚。本研究使用斑马鱼模型评估了慢性PFOS暴露对甲状腺结构和功能的影响。受精后8小时（hpf）的斑马鱼暴露于PFOS（250μg/l）中，直至受精后120天（dpf）。在受精后120天对斑马鱼的甲状腺激素（T和T）水平、甲状腺形态和甲状腺功能相关基因表达进行了评估。我们的研究结果表明，慢性PFOS暴露改变了甲状腺激素水平、甲状腺滤泡细胞结构和甲状腺激素相关基因表达，这表明斑马鱼作为PFOS慢性毒性筛选的替代模型是有效的。
