Bucukovski Joseph, Carter Jared A, Striemer Christopher C, Müllner Stefan, Schulte-Pelkum Johannes, Schulz-Knappe Peter, Miller Benjamin L
Adarza BioSystems, Inc., 2184 Welsch Industrial Court, St. Louis, MO 63146, United States.
Adarza BioSystems, Inc., 2184 Welsch Industrial Court, St. Louis, MO 63146, United States; Department of Dermatology, University of Rochester, Rochester, NY 14642, United States.
J Immunol Methods. 2018 Aug;459:44-49. doi: 10.1016/j.jim.2018.05.011. Epub 2018 May 24.
Multiplex assays for autoantibodies have shown utility both in research towards understanding the basic biology of autoimmune disease, and as tools for clinical diagnosis. New label-free multiplex analysis methods have the potential to streamline both the process of assay development and assay workflow. We report fabrication and testing of a 5-plex autoantigen microarray using the Arrayed Imaging Reflectometry (AIR) platform. This label-free technology provides rapid, sensitive, and quantitative detection of an arbitrary number of analytes in a standard multiwell format. In this work, we demonstrate that AIR is able to detect antibodies to Ro60, La/SSB, Scl-70, BicD2, and Ro52 in single-donor human serum samples with multiplex results comparable to singleplex ELISA or Luminex assays.
自身抗体的多重检测在理解自身免疫性疾病基本生物学的研究以及作为临床诊断工具方面均已显示出实用性。新的无标记多重分析方法有潜力简化检测开发过程和检测工作流程。我们报告了使用阵列成像反射测量法（AIR）平台制备和测试的5重自身抗原微阵列。这种无标记技术能以标准多孔板形式对任意数量的分析物进行快速、灵敏且定量的检测。在这项工作中，我们证明AIR能够在单供体人血清样本中检测抗Ro60、La/SSB、Scl-70、BicD2和Ro52的抗体，其多重检测结果与单重ELISA或Luminex检测相当。
