Department of Radiology and Biomedical Imaging, University of California, San Francisco, USA.
Department of Epidemiology and Biostatistics, University of California, San Francisco, USA.
Osteoarthritis Cartilage. 2018 Aug;26(8):1070-1077. doi: 10.1016/j.joca.2018.04.019. Epub 2018 May 23.
The purpose of this study was to assess the associations between serum/urine biomarkers for osteoarthritis and magnetic resonance (MR) imaging measures of cartilage composition and joint structure (cartilage, meniscus, and bone marrow), using MR imaging data from the Osteoarthritis Initiative (OAI).
141 subjects with Kellgren Lawrence (KL) grades 0-3 in the right knee and with available serum/urine biomarker assays were selected from the OAI. Cartilage magnetic resonance imaging (MRI) T2 measurements were performed in the medial femur, lateral femur, medial tibia, lateral tibia, and patella compartments. Compartment-specific knee morphologic grading [whole-organ magnetic resonance imaging score (WORMS)] in the cartilage, meniscus, and bone marrow was also performed. We focused on associations of serum hyaluronan (sHA), serum cartilage oligomeric matrix protein (sCOMP), serum matrix metalloproteinase-3 (sMMP3), and Urine Carboxy-Terminal Telepeptides of Type II Collagen (uCtX-II)) with MRI parameters (T2, WORMS), assessed using partial correlations adjusted for age, gender, body mass index (BMI), KL grade in both knees, and diabetes status.
Higher levels of sHA, sMMP3 and sCOMP were correlated (P < 0.05) with T2 of the lateral femur (r = 0.18 to 0.32) and lateral tibia (r = 0.17 to 0.23), and with average T2 of all knee regions (r = 0.23). uCTXII was correlated with patellar T2 (r = 0.19, P = 0.04). Among the morphologic measures, sHA and sMMP3 was positively correlated (r = 0.17 to 0.21, P < 0.05) with meniscal damage.
This study suggests weak, but statistically significant, correlations between serum biomarkers of OA (sHA, sCOMP, and sMMP3) and MRI T2 measures of cartilage extra-cellular matrix degeneration.
本研究旨在评估骨关节炎血清/尿液生物标志物与软骨成分和关节结构（软骨、半月板和骨髓）的磁共振（MR）成像测量值之间的相关性，所使用的数据来自于骨关节炎倡议（OAI）的 MR 成像数据。
从 OAI 中选择了右膝关节 Kellgren-Lawrence（KL）分级为 0-3 级且具有可用血清/尿液生物标志物检测结果的 141 名受试者。对内侧股骨、外侧股骨、内侧胫骨、外侧胫骨和髌骨进行了软骨磁共振成像（MRI）T2 测量。还对软骨、半月板和骨髓的关节整体磁共振成像评分（WORMS）进行了特定部位的膝关节形态学分级。我们重点关注血清透明质酸（sHA）、血清软骨寡聚基质蛋白（sCOMP）、血清基质金属蛋白酶-3（sMMP3）和尿型 II 胶原羧基末端肽（uCtX-II）与 MRI 参数（T2、WORMS）的相关性，使用偏相关分析进行评估，调整了年龄、性别、体重指数（BMI）、双膝 KL 分级和糖尿病状态的影响。
较高水平的 sHA、sMMP3 和 sCOMP 与外侧股骨（r=0.18 至 0.32）和外侧胫骨（r=0.17 至 0.23）的 T2 以及所有膝关节区域的平均 T2 呈正相关（r=0.23）。uCTX-II 与髌骨 T2 呈正相关（r=0.19，P=0.04）。在形态学测量中，sHA 和 sMMP3 与半月板损伤呈正相关（r=0.17 至 0.21，P<0.05）。
本研究表明，OA 的血清生物标志物（sHA、sCOMP 和 sMMP3）与软骨细胞外基质退变的 MRI T2 测量值之间存在微弱但具有统计学意义的相关性。
