Kurnakov Institute of General & Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 31, Moscow 119991, Russia.
Kurnakov Institute of General & Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 31, Moscow 119991, Russia.
J Chromatogr A. 2018 Jul 27;1560:26-34. doi: 10.1016/j.chroma.2018.05.032. Epub 2018 May 23.
Closed-loop recycling countercurrent chromatography (CLR CCC) with multiple sample injection has been shown to provide simultaneous concentration and separation of target compounds from multicomponent mixtures. Previous analysis of CLR CCC with multiple sample injections has been limited to the ideal recycling model, which neglects the effects caused by the pump and connecting lines. In this study, an analysis of the process is carried out based on the non-ideal recycling model: recycling chromatograms at two points of the closed-loop - the inlet of the column (A) and the outlet of the column (B) - are considered. The sample is repeatedly introduced at the inlet of the column when the circulating peak of target compound passes point A. Analytical expressions are developed, allowing the design and simulation of different variants of simultaneous separation and concentration of target compounds from multicomponent mixtures. Examples of separation of target compounds from three and five-component mixtures are discussed. Experimental results are presented demonstrating a reasonable agreement between the theory and the experiment. Due to its ability to concentrate individual solutes, CRL CCC with multiple sample injections can become an efficient analytical method to determine minor components in complex mixtures.
闭路循环逆流色谱（CLR CCC）与多次进样相结合，已被证明可从多组分混合物中同时浓缩和分离目标化合物。之前对多次进样的 CLR CCC 的分析仅限于理想的回收模型，该模型忽略了泵和连接管路引起的影响。在这项研究中，根据非理想的回收模型对该过程进行了分析：在闭环的两个点——柱入口（A）和柱出口（B）——处考虑循环色谱图。当目标化合物的循环峰通过点 A 时，在柱入口处重复引入样品。开发了分析表达式，允许设计和模拟从多组分混合物中同时分离和浓缩目标化合物的不同变体。讨论了从三组分和五组分混合物中分离目标化合物的示例。实验结果表明，理论与实验之间具有良好的一致性。由于其浓缩个别溶质的能力，多次进样的闭路循环逆流色谱（CLR CCC）可以成为一种从复杂混合物中测定微量成分的有效分析方法。
