Gorwill R H, Steele H D
Queen's University, Kingston, Ontario, Canada.
Reprod Toxicol. 1987;1(4):263-6. doi: 10.1016/0890-6238(87)90017-7.
Clomiphene citrate, administered to the neonatal mouse during cervicovaginal epithelial differentiation, is associated with subsequent adenosis. Similar lack of neonatal transformation of columnar to squamous epithelium occurs after diethylstilbestrol. Mouse neonatally exposed to diethylstilbestrol have a high incidence of reproductive tract tumors in later life. In this study Balb/c neonates were exposed to clomiphene citrate, maintained with controls, and killed at 52, 56, and 60 weeks. Cervicovaginal histology was studied by light microscopy. In all control animals only mature squamous epithelium was seen (44/44). After clomiphene citrate, at 52 weeks, 3/10 animals had other than mature squamous epithelium identified. One of these genital tracts contained adenosis. At 56 weeks only mature squamous epithelium was seen (11/11), and at 60 weeks immature squamous epithelium was identified in 1 of 10 animals. In no study animal (0/31) was there any evidence of tumor development. This differs from the high incidence of reproductive tract tumor seen in the mouse after neonatal diethylstilbestrol exposure.
在宫颈阴道上皮分化期间给新生小鼠施用枸橼酸氯米芬，会导致随后发生腺病。己烯雌酚使用后也会出现类似的柱状上皮向鳞状上皮的新生儿期转化缺失。新生期接触己烯雌酚的小鼠在以后的生活中有很高的生殖道肿瘤发生率。在本研究中，将Balb/c新生小鼠暴露于枸橼酸氯米芬，同时设置对照组，在52、56和60周时处死小鼠。通过光学显微镜研究宫颈阴道组织学。在所有对照动物中，仅可见成熟的鳞状上皮（44/44）。在使用枸橼酸氯米芬后，在52周时，10只动物中有3只被鉴定为具有非成熟鳞状上皮。其中一个生殖道含有腺病。在56周时仅可见成熟的鳞状上皮（11/11），在60周时，10只动物中有1只被鉴定为具有未成熟的鳞状上皮。在任何研究动物中（0/31）均未发现肿瘤发生的证据。这与新生期接触己烯雌酚的小鼠中所见的高发生率生殖道肿瘤不同。
