lncRNA-HULC 多态性与肝细胞癌风险和预后的关联。
The association of lncRNA-HULC polymorphisms with hepatocellular cancer risk and prognosis.
机构信息
Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang 110001, China; Hepatobiliary Surgery Department of General Surgery Institute, the First Hospital of China Medical University, Shenyang 110001, China.
Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang 110001, China.
出版信息
Gene. 2018 Sep 5;670:148-154. doi: 10.1016/j.gene.2018.05.096. Epub 2018 May 24.
BACKGROUND
Genetic polymorphisms in lncRNA HULC may affect the susceptibility and clinical outcome of cancer. We aimed to investigate the association of HULC tagSNPs with the risk and prognosis of hepatocellular cancer, as well as the influence of the SNPs on lncRNA expression level.
METHODS
A total of 1338 samples were recruited in the risk study. Among them, 351 HCC patients were involved in the prognosis study. SNP genotyping was performed using KASP method and lncRNA expression was detected by Real-time PCR.
RESULTS
We found a promoter SNP, rs1041279, was associated with a 1.41-fold increased HCC risk (P = 0.032). In the stratified analysis, rs1041279 had greater ORs for the increased HCC risk in the male subgroup (P = 0.014, OR = 1.54). Furthermore, multi-logistic regression analysis revealed a two-way interaction effect of smoking-rs2038540 SNP on HCC risk (OR = 4.20). And MDR analysis consistently demonstrated a SNP-environmental interaction among smoking-drinking-rs2038540 SNP as the best model for predicting HCC risk (P = 0.0107). In our study, no significant association was found between HULC SNPs and the overall survival (P > 0.05), and no significant effect was observed of rs1041279 SNP on lncRNA-HULC expression (P > 0.05).
CONCLUSION
lncRNA-HULC rs1041279 SNP and the interaction of rs2038540 SNP with environmental factors could enhance HCC risk.
背景
lncRNA HULC 的遗传多态性可能影响癌症的易感性和临床结局。我们旨在研究 HULC 标签 SNP 与肝细胞癌风险和预后的关系，以及 SNP 对 lncRNA 表达水平的影响。
方法
共纳入 1338 例样本进行风险研究，其中 351 例 HCC 患者纳入预后研究。采用 KASP 方法进行 SNP 基因分型，实时 PCR 检测 lncRNA 表达。
结果
我们发现启动子 SNP rs1041279 与 HCC 风险增加 1.41 倍相关（P=0.032）。分层分析显示，rs1041279 与男性亚组 HCC 风险增加的 OR 更大（P=0.014，OR=1.54）。此外，多元逻辑回归分析显示，吸烟-rs2038540 SNP 之间存在双向交互作用，对 HCC 风险的影响（OR=4.20）。MDR 分析一致表明，吸烟-饮酒-rs2038540 SNP 之间存在 SNP-环境相互作用，是预测 HCC 风险的最佳模型（P=0.0107）。在本研究中，我们未发现 HULC SNP 与总生存期之间存在显著相关性（P＞0.05），也未观察到 rs1041279 SNP 对 lncRNA-HULC 表达的显著影响（P＞0.05）。
结论
lncRNA-HULC rs1041279 SNP 以及 rs2038540 SNP 与环境因素的相互作用可能增加 HCC 风险。
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