Lu Yan-Ming, Guo Ya-Ru, Zhou Meng-Ya, Wang Yue
Department of Gynecology and Obstetrics, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110003, P.R. China.
Exp Ther Med. 2022 Jun;23(6):408. doi: 10.3892/etm.2022.11335. Epub 2022 Apr 26.
Ovarian cancer (OC) is a highly lethal disease among all gynecologic malignant tumor types. Accumulating studies have indicated that certain long non-coding RNAs (lncRNAs) serve important roles in the development and progression of OC. In a previous study by our group, lncRNA BC041954 was identified as one of the most upregulated lncRNAs in OC. In the present study, the clinical significance of lncRNA BC041954 in OC was evaluated. The expression of BC041954 was detected in OC and non-tumor tissue (NT) samples using reverse transcription-quantitative PCR. Furthermore, the association between BC041954 and clinicopathological variables was analyzed using the Chi-square test. Survival was determined using Kaplan-Meier analysis. The prognostic significance of BC041954 was evaluated using univariate and multivariate logistic regression analyses. MicroRNA (miRNA)-lncRNA and miRNA-mRNA pairs were used to construct the lncRNA-miRNA-mRNA competing endogenous RNA network with an in-house Perl script. BC041954 expression was increased in 103 OC tissues as compared with that in NT tissues. Upregulated BC041954 expression was significantly associated with the International Federation of Gynecology and Obstetrics stage and distant metastasis. Kaplan-Meier analysis demonstrated that patients with high BC041954 expression had lower overall survival (OS). In the multivariate logistic regression analysis, BC041954 was also identified as an independent poor prognostic factor for OS in patients with OC. The results suggested that overexpression of the lncRNA BC041954 is a poor prognostic indicator in patients with OC.
在所有妇科恶性肿瘤类型中，卵巢癌（OC）是一种致死率很高的疾病。越来越多的研究表明，某些长链非编码RNA（lncRNA）在OC的发生和发展过程中发挥着重要作用。在我们团队之前的一项研究中，lncRNA BC041954被确定为OC中上调最为明显的lncRNA之一。在本研究中，对lncRNA BC041954在OC中的临床意义进行了评估。使用逆转录定量PCR检测OC和非肿瘤组织（NT）样本中BC041954的表达。此外，采用卡方检验分析BC041954与临床病理变量之间的关联。使用Kaplan-Meier分析确定生存率。采用单因素和多因素逻辑回归分析评估BC041954的预后意义。使用内部Perl脚本构建lncRNA- miRNA- mRNA竞争性内源RNA网络的miRNA- lncRNA和miRNA- mRNA对。与NT组织相比，103例OC组织中BC041954表达升高。BC041954表达上调与国际妇产科联合会分期和远处转移显著相关。Kaplan-Meier分析表明，BC041954高表达的患者总生存期（OS）较低。在多因素逻辑回归分析中，BC041954也被确定为OC患者OS的独立不良预后因素。结果表明，lncRNA BC041954的过表达是OC患者不良预后的指标。
