Department of Periodontology, Jinan Stomatological Hospital, Jinan, Shandong, China.
Department of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong, China.
Environ Sci Pollut Res Int. 2018 Aug;25(22):22205-22212. doi: 10.1007/s11356-018-2300-z. Epub 2018 May 26.
Long non-coding RNAs (lncRNAs) have gained a lot of attention because they participate in several human disorders, including tumors. This study determined the role of LncRNA CASC15 (cancer susceptibility candidate 15) in the development of tongue squamous cell carcinoma (TSCC). Here, we identified that CASC15 expression was upregulated in TSCC samples and cell lines. We showed that overexpression of CASC15 promoted cell proliferation, cycle, and migration in TSCC. In addition, we revealed that miR-33a-5p expression was downregulated in TSCC tissues and cell lines. Moreover, we showed that the expression of CASC15 was negatively related with miR-33a-5p expression in TSCC tissues. Ectopic expression of miR-33a-5p suppressed cell proliferation, cycle, and migration in TSCC. Elevated expression of CASC15 suppressed miR-33a-5p expression and promoted ZEB1 expression in SCC4 cell. Ectopic expression of CASC15 promoted TSCC cell proliferation, cycle, and migration through targeting miR-33a-5p. These results suggested that lncRNA CASC15 and miR-33a-5p might be exploited as new markers of TSCC and were potential treatment targets for TSCC patients.
长链非编码 RNA（lncRNA）因其参与多种人类疾病，包括肿瘤，而受到广泛关注。本研究旨在确定 LncRNA CASC15（癌症易感性候选基因 15）在舌鳞状细胞癌（TSCC）发展中的作用。在这里，我们发现 CASC15 在 TSCC 样本和细胞系中表达上调。我们表明，CASC15 的过表达促进了 TSCC 中的细胞增殖、周期和迁移。此外，我们揭示了 miR-33a-5p 在 TSCC 组织和细胞系中的表达下调。此外，我们还表明，在 TSCC 组织中，CASC15 的表达与 miR-33a-5p 的表达呈负相关。miR-33a-5p 的过表达抑制了 TSCC 中的细胞增殖、周期和迁移。CASC15 的高表达抑制了 SCC4 细胞中 miR-33a-5p 的表达，并促进了 ZEB1 的表达。CASC15 的过表达通过靶向 miR-33a-5p 促进了 TSCC 细胞的增殖、周期和迁移。这些结果表明，lncRNA CASC15 和 miR-33a-5p 可能被用作 TSCC 的新标志物，并可能成为 TSCC 患者的潜在治疗靶点。
