Li Xin, Bau Tolgor, Bao Haiying
Engineering Research Centre of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China.
Oncol Lett. 2018 Jun;15(6):8357-8362. doi: 10.3892/ol.2018.8380. Epub 2018 Mar 29.
In the present study, the triterpenoid 3-acetoxylanosta-8,24-dien-21-oic acid (FPOA) was extracted from . The aim of the present was to elucidate the mechanism of action of FPOA in HeLa cervical cancer cells. Cell viability was examined using an MTT assay and the morphological detection of apoptosis was conducted using DAPI staining. The rate of apoptosis was examined via Annexin V-FITC/PI double staining and the expression levels of apoptosis-associated proteins were determined by western blot analysis. FPOA was observed to inhibit HeLa cell proliferation, with IC values of 25.28, 15.30 and 11.79 µg/ml at 24, 48 and 72 h, respectively. Typical apoptotic bodies were observed in the HeLa cells following treatment with FPOA, as revealed by DAPI staining. The percentage of apoptotic cells was 3.00, 3.12, 6.18 and 32.28% following treatment with FPOA at concentrations of 0, 7.5, 15 and 30 µg/ml, respectively. Western blot analysis showed that caspase-3 and -9 were cleaved more frequently after treatment with FPOA. Furthermore, the expression of Bax was increased but Bcl-2 expression was decreased after treatment with FPOA. These results suggest that FPOA can induce HeLa cell apoptosis through a caspase-mediated pathway.
在本研究中，三萜类化合物3-乙酰氧基羊毛甾-8,24-二烯-21-酸（FPOA）是从……中提取的。本研究的目的是阐明FPOA对人宫颈癌HeLa细胞的作用机制。采用MTT法检测细胞活力，用DAPI染色进行凋亡的形态学检测。通过Annexin V-FITC/PI双染法检测凋亡率，并用蛋白质免疫印迹分析确定凋亡相关蛋白的表达水平。观察到FPOA可抑制HeLa细胞增殖，在24、48和72小时时的半数抑制浓度（IC）值分别为25.28、15.30和11.79μg/ml。DAPI染色显示，用FPOA处理后的HeLa细胞中观察到典型的凋亡小体。用浓度为0、7.5、15和30μg/ml的FPOA处理后，凋亡细胞的百分比分别为3.00%、3.12%、6.18%和32.28%。蛋白质免疫印迹分析表明，用FPOA处理后，caspase-3和-9的切割更为频繁。此外，用FPOA处理后，Bax的表达增加，但Bcl-2的表达减少。这些结果表明，FPOA可通过caspase介导的途径诱导HeLa细胞凋亡。
