Department of Chemical Engineering , Indian Institute of Technology (IIT) Delhi , New Delhi 110016 , India.
Langmuir. 2018 Jun 26;34(25):7396-7403. doi: 10.1021/acs.langmuir.8b00978. Epub 2018 Jun 12.
Detection and removal of lipopolysaccharides (LPS) from food and pharmaceutical preparations is important for their safe intake and administration to avoid septic shock. We have developed an abiotic system for reversible capture, removal, and detection of LPS in aqueous solutions. Our system comprises long C18 acyl chains tethered to FeO/Au/FeO nanoflowers (NFs) that act as solid supports during the separation process. The reversible LPS binding is mediated by facile hydrophobic interactions between the C18 chains and the bioactive lipid A component present on the LPS molecule. Various parameters such as pH, solvent, sonication time, NF concentration, alkane chain length, and density are optimized to achieve a maximum LPS capture efficiency. The NFs can be reused at least three times by simply breaking the NF-LPS complexes in the presence of food-grade surfactants, making the entire process safe, efficient, and scalable. The regenerated particles also serve as colorimetric labels in dot blot bioassays for simple and rapid estimation of the LPS removed.
从食品和药物制剂中检测和去除脂多糖 (LPS) 对于安全摄入和给药以避免败血症性休克非常重要。我们开发了一种非生物系统，用于可逆地捕获、去除和检测水溶液中的 LPS。我们的系统包括长 C18 酰基链，它们被固定在 FeO/Au/FeO 纳米花 (NFs) 上，在分离过程中充当固体支撑物。LPS 结合的可逆性是通过 C18 链与 LPS 分子上存在的生物活性脂质 A 成分之间的简单疏水相互作用介导的。优化了各种参数，如 pH 值、溶剂、超声时间、NF 浓度、烷链长度和密度，以实现最大的 LPS 捕获效率。通过在存在食品级表面活性剂的情况下简单地打破 NF-LPS 复合物，NF 可以至少重复使用三次，从而使整个过程安全、高效且可扩展。再生颗粒还可用作斑点印迹生物测定中的比色标记物，用于简单快速估计去除的 LPS。
