School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Longkun Rd. 99, Hainan 570100, China.
Int J Biol Macromol. 2018 Oct 1;117:878-889. doi: 10.1016/j.ijbiomac.2018.05.180. Epub 2018 May 26.
In this study, a Se-deficient mice model was successfully developed by feeding a Se-deficient diet (0.02 mg Se/kg diet) for 4 weeks, and Se supplementation by Se-polysaccharides (Se-GFP-22) was lasted for 4 weeks. The immunomodulatory activity and Se supplementation of Se-GFP-22 from Se-enriched G. frondosa was investigated. Results showed that Se-GFP-22 remarkably enhanced glutathione peroxidase (GSH-Px) and thioredoxin reductase (TrxR) activities in liver, kidney and plasma, and serum, liver, spleen and kidney Se levels of Se-deficient mice. Se-GFP-22 increased the thymus and spleen indices, phagocytic index, co-mitogenic (ConA- or LPS-stimulated) activities on splenocytes and DTH reaction. Se-GFP-22 caused significant increments in cytokine (IL-1β, TNF-α and IFN-γ) levels and Ig G, Ig A, Ig M and Ig E levels. Se-GFP-22 exhibited superior immunomodulatory effects than GFP-22. These findings indicated that Se-GFP-22 promote the protective effects against Se deficiency-induced immunosuppression and could be a potential immunomodulatory agent and a dietary Se-supplement.
在这项研究中，通过喂食含硒量为 0.02mg/kg 饮食的缺硒饮食 4 周成功建立了 Se 缺乏的小鼠模型，并通过 Se-多糖（Se-GFP-22）进行了 4 周的 Se 补充。研究了从富硒灵芝中提取的 Se-GFP-22 的免疫调节活性和 Se 补充作用。结果表明，Se-GFP-22 显著提高了 Se 缺乏小鼠肝脏、肾脏和血浆以及血清、肝脏、脾脏和肾脏中的谷胱甘肽过氧化物酶（GSH-Px）和硫氧还蛋白还原酶（TrxR）活性。Se-GFP-22 增加了胸腺和脾脏指数、吞噬指数、脾细胞协同刺激（ConA 或 LPS 刺激）活性和迟发型超敏反应。Se-GFP-22 导致细胞因子（IL-1β、TNF-α 和 IFN-γ）水平和 IgG、IgA、IgM 和 IgE 水平显著增加。Se-GFP-22 表现出比 GFP-22 更优异的免疫调节作用。这些发现表明，Se-GFP-22 促进了对 Se 缺乏引起的免疫抑制的保护作用，并且可能是一种潜在的免疫调节剂和膳食 Se 补充剂。
