Lauková Andrea, Kubašová Ivana, Kandričáková Anna, Strompfová Viola, Žitňan Rudolf, Simonová Monika Pogány
Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
National Agricultural and Food Centre, Hlohovecká 2, 951 41 Nitra-Lužianky, Košice, Slovakia.
Folia Microbiol (Praha). 2018 Nov;63(6):749-755. doi: 10.1007/s12223-018-0616-1. Epub 2018 May 28.
Aeromonads represent bacteria thought to be primarily mostly autochthonous to aquatic environments. This study was focused on the relation with antibiotics and enterocins of identified Aeromonas species isolated from the intestine of trouts living in Slovakian aquatic sources. Intestinal samples from 50 trouts (3 Salmo trutta and 47 Salmo gairdnerii) were collected in April of years 2007, 2010, and 2015 from trouts of different water sources in Slovakia (pond Bukovec near Košice, river Čierny Váh). Due to the MALDI-TOF mass spectrometry evaluation, 25 strains were proposed to the genus Aeromonas involving nine different species (Aeromonas bestiarum-nine strains, Aer. salmonicida-four strains, Aer. encheleia, Aer. eucrenophila, Aer. molluscorum, Aer. media, Aer. sobria, Aer. popoffii, Aer. veronii). Phenotypic evaluation of individual strains confirmed their species identification. Twenty-five strains of different Aeromonas species were sensitive to azithromycin, amikacin, mecillinam, mezlocillin, piperacillin, gentamicin, chloramphenicol, and tetracycline. On the other side, they were resistant to carbenicillin and ticarcillin. The growth of Aer. bestiarum R41/1 was inhibited by treatment with Ent M and Ent 2019 (inhibition activity 100 AU/mL). Aer. bestiarum R47/3 was inhibited by eight enterocins (100 AU/mL). It is the first study testing enterocins to inhibit the growth of Aeromonas species from trouts.
气单胞菌是一类主要被认为原产于水生环境的细菌。本研究聚焦于从斯洛伐克水生环境中生活的鳟鱼肠道分离出的已鉴定气单胞菌属物种与抗生素和肠菌素的关系。2007年、2010年和2015年4月，从斯洛伐克不同水源（科希策附近的布科韦茨池塘、黑瓦赫河）的50条鳟鱼（3条褐鳟和47条虹鳟）采集肠道样本。通过基质辅助激光解吸电离飞行时间质谱评估，25株菌株被鉴定为气单胞菌属，涉及9个不同物种（嗜兽气单胞菌9株、杀鲑气单胞菌4株、鳗气单胞菌、真嗜泉气单胞菌、软体动物气单胞菌、中间气单胞菌、温和气单胞菌、波氏气单胞菌、维氏气单胞菌）。对各个菌株的表型评估证实了它们的物种鉴定。25株不同气单胞菌属物种的菌株对阿奇霉素、阿米卡星、美西林、美洛西林、哌拉西林、庆大霉素、氯霉素和四环素敏感。另一方面，它们对羧苄西林和替卡西林耐药。嗜兽气单胞菌R41/1的生长受到Ent M和Ent 2019处理的抑制（抑制活性为100 AU/mL）。嗜兽气单胞菌R47/3受到8种肠菌素（100 AU/mL）的抑制。这是第一项测试肠菌素抑制鳟鱼气单胞菌属物种生长的研究。
