Du Xiaoli, Wang Mengyu, Zhou Haijian, Li Zhenpeng, Xu Jialiang, Li Zhe, Kan Biao, Chen Daoli, Wang Xiaoli, Jin Yujuan, Ren Yan, Ma Yanping, Liu Jiuyin, Luan Yang, Cui Zhigang, Lu Xin
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
School of Public Health, Nanchang University, Nanchang, Jiangxi, China.
Front Microbiol. 2021 Jan 18;11:625961. doi: 10.3389/fmicb.2020.625961. eCollection 2020.
We compared several identification methods for genus members, including traditional biochemical testing, multiplex-PCR amplification, mass spectrometry identification, whole-genome sequencing, multilocus phylogenetic analysis (MLPA), and , and - gene sequencing. Isolates ( = 62) belonging to the genus, which were came from the bacterial bank in the laboratory, were used to assess the identification accuracy of the different methods. Whole-genome sequencing showed that the spp. isolates comprised ( = 21), ( = 18), ( = 8), ( = 7), ( = 5), ( = 2), and ( = 1). Using the whole-genome sequencing results as the standard, the consistency of the other methods was compared with them. The results were 46.77% (29/62) for biochemical identification, 83.87% (52/62) for mass spectrometric identification, 67.74% (42/62) for multiplex-PCR, 100% (62/62) for MLPA typing, 72.58% for , and 59.68% for and -. MLPA was the most consistent, followed by mass spectrometry. Therefore, in the public health laboratory, both MLPA and whole-genome sequencing methods can be used to identify various species. However, rapid and relatively accurate mass spectrometry is recommended for clinical lab.
我们比较了几种用于该属成员的鉴定方法，包括传统生化检测、多重聚合酶链反应（multiplex-PCR）扩增、质谱鉴定、全基因组测序、多位点系统发育分析（MLPA）以及[具体基因名称1]和[具体基因名称2]基因测序。从实验室细菌库中获取的属于该属的62株分离株用于评估不同方法的鉴定准确性。全基因组测序显示，该属的分离株包括[具体种名1]（21株）、[具体种名2]（18株）、[具体种名3]（8株）、[具体种名4]（7株）、[具体种名5]（5株）、[具体种名6]（2株）和[具体种名7]（1株）。以全基因组测序结果作为标准，将其他方法的一致性与之进行比较。生化鉴定的一致性为46.77%（29/62），质谱鉴定为83.87%（52/62），多重聚合酶链反应为67.74%（42/62），MLPA分型为100%（62/62），[具体基因名称1]基因测序为72.58%，[具体基因名称2]和[具体基因名称3]基因测序为59.68%。MLPA的一致性最高，其次是质谱。因此，在公共卫生实验室中，MLPA和全基因组测序方法均可用于鉴定该属的各种物种。然而，对于临床实验室，建议使用快速且相对准确的质谱法。
