基于双标记的快速灵敏免疫层析法检测谷物中的玉米赤霉烯酮

Rapid and sensitive double-label based immunochromatographic assay for zearalenone detection in cereals.

作者信息

Wang Du, Zhang Zhaowei, Zhang Qi, Wang Zhongzheng, Zhang Wen, Yu Li, Li Hui, Jiang Jun, Li Peiwu

机构信息

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, P. R. China.

National Reference Laboratory for Biotoxin Test, Wuhan, P. R. China.

出版信息

Electrophoresis. 2018 Aug;39(16):2125-2130. doi: 10.1002/elps.201800055.

DOI:10.1002/elps.201800055

PMID:29808596

Abstract

A double-label immunochromatographic based assay (DL-ICA) was developed to monitor zearalenone (ZEN) levels in cereals, based on Eu nanoparticles (EuNP). The DL-ICA exhibited excellent sensitivity, reliability and selectivity in real samples. It showed low limits of detection (0.21-0.25 μg/kg) and broad analytical ranges (up to 120 μg/kg). The total analytical time, including sample preparation and DL-ICA execution, was reduced by 15 min compared with HPLC. The recovery rates ranged from 95.0-118.4%, with relative standard deviations (RSD) <11.6%. Inter- and intra-day validations were assessed, recovery rates of 89.3-106.9% and RSD of 2.3-9.7% were obtained, suggesting considerable stability and reliability for the assay. An excellent correlation was observed between DL-ICA and a reference HPLC method (R = 0.9899). Compared to current immunoassays, the current DL-ICA is inexpensive, highly sensitive, and rapid. Therefore, DL-ICA constitutes a novel tool for monitoring mycotoxins in food and feed to ensure safety.

摘要

基于铕纳米颗粒（EuNP）开发了一种双标记免疫色谱分析法（DL-ICA），用于监测谷物中的玉米赤霉烯酮（ZEN）水平。DL-ICA在实际样品中表现出优异的灵敏度、可靠性和选择性。它具有低检测限（0.21 - 0.25μg/kg）和宽分析范围（高达120μg/kg）。与高效液相色谱法（HPLC）相比，包括样品制备和执行DL-ICA在内的总分析时间减少了15分钟。回收率在95.0 - 118.4%之间，相对标准偏差（RSD）<11.6%。进行了日间和日内验证，回收率为89.3 - 106.9%，RSD为2.3 - 9.7%，表明该分析方法具有相当的稳定性和可靠性。DL-ICA与参考HPLC方法之间观察到极好的相关性（R = 0.9899）。与当前的免疫分析方法相比，当前的DL-ICA价格低廉、灵敏度高且快速。因此，DL-ICA构成了一种监测食品和饲料中霉菌毒素以确保安全的新型工具。