三种基于纳米颗粒标记物的侧向流动免疫层析分析方法用于荧光法测定玉米赤霉烯酮。
Three kinds of lateral flow immunochromatographic assays based on the use of nanoparticle labels for fluorometric determination of zearalenone.
机构信息
College of Food Engineering and Biotechnology Tianjin University of Science and Technology, Tianjin Engineering Centre of Food Safety Control and Technology, Tianjin, 300457, China.
Medical College Nankai University, Tianjin, 300500, China.
出版信息
Mikrochim Acta. 2018 Mar 24;185(4):238. doi: 10.1007/s00604-018-2778-6.
Colloidal gold, quantum dots and polystyrene microspheres were used as labels in three kinds of lateral flow immunochromatographic assays (ICAs) for the detection of zearalenone (ZEN) in cereal samples. The assays allow ZEN to be quantified within 20 min. The LODs are 10 μg·L of ZEN for the colloidal gold-based ICA, and 1 μg·L for both the quantum dot and polystyrene microsphere based ICAs. The respective data are 60 μg·kg, 6 μg·kg and 6 μg·kg, respectively, for spiked samples and cereals. Only minor cross-sensitivity occurred between ZEN and fusarium toxins, and no cross-sensitivity if found for aflatoxin B, T-2 mycotoxin, ochratoxin A, deoxynivalenol, and fumonisin B. LODs of the three assays are lower than the maximum limits of ZEN set by most standardization agencies. Graphical abstract Schematic presentation of three lateral flow immunochromatographic assays (ICAs) based on the use of (a) colloidal gold (CG), (b) fluorescent quantum dots (QD), and
胶体金、量子点和聚苯乙烯微球被用作三种侧向流动免疫层析分析（ICAs）检测谷物样品中玉米赤霉烯酮（ZEN）的标记物。这些分析方法可在 20 分钟内定量 ZEN。基于胶体金的 ICA 的 LOD 为 10μg·L，基于量子点和聚苯乙烯微球的 ICA 的 LOD 均为 1μg·L。对于添加的样品和谷物，分别为 60μg·kg、6μg·kg 和 6μg·kg。ZEN 和镰刀菌毒素之间只有轻微的交叉敏感性，如果发现黄曲霉毒素 B、T-2 真菌毒素、赭曲霉毒素 A、脱氧雪腐镰刀菌烯醇和伏马菌素 B，则没有交叉敏感性。这三种检测方法的 LOD 均低于大多数标准化机构设定的 ZEN 最大限量。 图表摘要 三种基于（a）胶体金（CG）、（b）荧光量子点（QD）和
Zotero 插件