循环 microRNA-126 水平的变化与急性哮喘患儿免疫失衡有关。
Changes in circulating microRNA-126 levels are associated with immune imbalance in children with acute asthma.
机构信息
1 Department of Respiratory, Children's Hospital of Nanjing Medical University, Nanjing, P.R. China.
2 Department of Cardiothoracic Surgery, The Affiliated Wuxi People's Hospital, Nanjing Medical University, Wuxi, P.R. China.
出版信息
Int J Immunopathol Pharmacol. 2018 Jan-Dec;32:2058738418779243. doi: 10.1177/2058738418779243.
Regulation of the immune response in asthma is complex. MicroRNA-126 (miR-126) expression has been implicated in this response, so we sought to determine the clinical significance of miR-126 measured in the peripheral blood. A total of 80 children with acute asthma were selected to participate in the study and were compared to 80 healthy children. The relative circulating miR-126 levels, interleukin (IL)-4 levels, and the Th17 cell percentage in the peripheral blood of children in the case group were significantly higher than those in the control group, while the interferon (IFN)-γ levels and the CD4CD25Treg cell percentage were significantly lower than those in the control group. Along with the aggravation of the disease, the relative levels of miR-126 and IL-4 and the percentage of Th17 cells increased gradually, while the IFN-γ levels and the CD4CD25Treg cell percentage decreased. The relative level of miR-126 in the peripheral blood of children with asthma was positively correlated with IL-4 and the Th17 cell percentage and was negatively correlated with IFN-γ levels, CD4CD25Treg cell percentage and lung function indicators. The relative level of miR-126 was correlated with the Th17 cell percentage in the peripheral blood, forced vital capacity (FVC), and forced expiratory flow (FEF) of the children with asthma. The relative levels of miR-126 and IL-4 and the Th17 cell percentage were positively correlated with the severity of the asthma, while IFN-γ levels and the CD4CD25Treg cell percentage were negatively correlated with the severity of the asthma. CD4CD25Treg cell percentage and relative miR-126 levels were of the most predictive value in the diagnosis of asthma. Our findings show that the overexpression of miR-126 in acute asthma is correlated with signs of immune imbalance and is predictive of the severity of the disease, suggesting that it could be used as a potential serological marker for asthma diagnosis and evaluation.
哮喘的免疫反应调节复杂。miR-126（miR-126）的表达与这种反应有关，因此我们试图确定外周血中测量的 miR-126 的临床意义。总共选择了 80 名患有急性哮喘的儿童参与研究，并与 80 名健康儿童进行了比较。病例组儿童外周血中相对循环 miR-126 水平、白细胞介素（IL）-4 水平和 Th17 细胞百分比明显高于对照组，而干扰素（IFN）-γ水平和 CD4CD25Treg 细胞百分比明显低于对照组。随着疾病的加重，miR-126 和 IL-4 的相对水平以及 Th17 细胞的百分比逐渐增加，而 IFN-γ水平和 CD4CD25Treg 细胞的百分比则降低。哮喘患儿外周血中 miR-126 的相对水平与 IL-4 和 Th17 细胞百分比呈正相关，与 IFN-γ水平、CD4CD25Treg 细胞百分比和肺功能指标呈负相关。miR-126 与哮喘患儿外周血中的 Th17 细胞百分比、用力肺活量（FVC）和用力呼气流量（FEF）相关。miR-126 和 IL-4 的相对水平以及 Th17 细胞百分比与哮喘的严重程度呈正相关，而 IFN-γ水平和 CD4CD25Treg 细胞百分比与哮喘的严重程度呈负相关。CD4CD25Treg 细胞百分比和相对 miR-126 水平在哮喘的诊断中具有最高的预测价值。我们的研究结果表明，急性哮喘中 miR-126 的过度表达与免疫失衡的迹象相关，并且可以预测疾病的严重程度，这表明它可能作为哮喘诊断和评估的潜在血清学标志物。
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