采用高效液相色谱法研究血浆中白三烯的代谢。

The metabolism of leukotrienes in blood plasma studied by high-performance liquid chromatography.

作者信息

Köller M, Schönfeld W, Knöller J, Bremm K D, König W, Spur B, Crea A, Peters W

出版信息

Biochim Biophys Acta. 1985 Jan 9;833(1):128-34. doi: 10.1016/0005-2760(85)90260-7.

Abstract

The metabolism of leukotrienes (B4, C4, D4, and E4) within human plasma was studied and a simple sample preparation is presented. It was demonstrated that leukotriene E4 and leukotriene B4 were stable during incubation at 37 degrees C using the in vitro system. In contrast, leukotriene C4 was metabolized by gamma-glutamyl transpeptidase activities into leukotriene D4 which was further metabolized by dipeptidase activities of plasma into leukotriene E4. The transition state inhibitor of gamma-glutamyl transpeptidase L-serine-borate decreased the metabolism of leukotriene C4 in plasma. Dilution of plasma demonstrated that the dipeptidase was more active compared to the gamma-glutamyl transpeptidase. The metabolizing activities of plasma were functionally characterized by fractionating the plasma proteins.

摘要

对人血浆中白三烯（B4、C4、D4和E4）的代谢进行了研究，并介绍了一种简单的样品制备方法。结果表明，使用体外系统时，白三烯E4和白三烯B4在37℃孵育期间是稳定的。相比之下，白三烯C4被γ-谷氨酰转肽酶活性代谢为白三烯D4，而白三烯D4又被血浆中的二肽酶活性进一步代谢为白三烯E4。γ-谷氨酰转肽酶的过渡态抑制剂L-丝氨酸-硼酸盐降低了血浆中白三烯C4的代谢。血浆稀释表明，二肽酶比γ-谷氨酰转肽酶更具活性。通过对血浆蛋白进行分级分离，对血浆的代谢活性进行了功能表征。