心房利钠因子对肾上腺皮质细胞膜腺苷酸环化酶活性的抑制作用。

Inhibitory effect of atrial natriuretic factor on adenylate cyclase activity in adrenal cortical membranes.

作者信息

Anand-Srivastava M B, Genest J, Cantin M

出版信息

FEBS Lett. 1985 Feb 25;181(2):199-202. doi: 10.1016/0014-5793(85)80259-3.

Abstract

The effect of rat synthetic atrial natriuretic factor (ANF) on adenylate cyclase activity was studied in adrenal cortical membranes. Synthetic ANF (Arg 101-Tyr 126) inhibited adenylate cyclase activity in a concentration-dependent manner. The maximum inhibition observed was about 25% with an apparent ki of 5 X 10(-11) to 10(-10) M. Various hormones such as isoproterenol, dopamine, prostaglandin (PGE1) and ACTH-stimulated adenylate cyclase to various degrees and ANF inhibited the stimulatory effect of all these hormones to some degree but never abolished it. In addition, ANF was also able to inhibit the stimulation exerted by forskolin which activates adenylate cyclase by receptor-independent mechanism. This is the first study demonstrating the inhibitory effect of ANF on adrenal cortical adenylate cyclase. From these results it can be suggested that the inhibition of adenylate cyclase may be one of the mechanisms through which ANF exerts the inhibitory effect on steroidogenesis stimulated by various hormones and agents.

摘要

在肾上腺皮质膜中研究了大鼠合成心房利钠因子（ANF）对腺苷酸环化酶活性的影响。合成ANF（精氨酸101 - 酪氨酸126）以浓度依赖的方式抑制腺苷酸环化酶活性。观察到的最大抑制率约为25%，表观解离常数（ki）为5×10⁻¹¹至10⁻¹⁰M。各种激素如异丙肾上腺素、多巴胺、前列腺素（PGE1）和促肾上腺皮质激素（ACTH）能不同程度地刺激腺苷酸环化酶，而ANF在一定程度上抑制所有这些激素的刺激作用，但从未完全消除。此外，ANF还能够抑制福斯高林（forskolin）所产生的刺激作用，福斯高林通过非受体依赖机制激活腺苷酸环化酶。这是首次证明ANF对肾上腺皮质腺苷酸环化酶具有抑制作用的研究。从这些结果可以推测，抑制腺苷酸环化酶可能是ANF对各种激素和药物刺激的类固醇生成发挥抑制作用的机制之一。