Anand-Srivastava M B, Franks D J, Cantin M, Genest J
Biochem Biophys Res Commun. 1984 Jun 29;121(3):855-62. doi: 10.1016/0006-291x(84)90756-3.
The synthetic atrial natriuretic factor (ANF) (8- 33AA ) inhibited adenylate cyclase activity in aorta washed particles, mesenteric artery, and renal artery homogenates in a concentration dependent manner with an apparent Ki between 0.1 to 1nM . The extent of inhibition of adenylate cyclase by ANF varied from tissue to tissue. The adenylate cyclase from mesenteric artery and renal artery was inhibited to a greater extent as compared to that from aorta. ANF was also able to inhibit the stimulatory effects of hormones on adenylate cyclase activity and of agents such as F- and forskolin which activate adenylate cyclase by receptor- independent mechanism. In addition, ANF showed an additive effect with the inhibitory response of angiotensin II on adenylate cyclase from rat aorta. These studies for the first time demonstrate that ANF is an inhibitor of adenylate cyclase of several systems.
合成心房利钠因子（ANF）（8 - 33AA）以浓度依赖方式抑制主动脉洗涤颗粒、肠系膜动脉和肾动脉匀浆中的腺苷酸环化酶活性，其表观解离常数（Ki）介于0.1至1纳摩尔之间。ANF对腺苷酸环化酶的抑制程度因组织而异。与主动脉相比，肠系膜动脉和肾动脉中的腺苷酸环化酶受到的抑制程度更大。ANF还能够抑制激素对腺苷酸环化酶活性的刺激作用，以及诸如氟化物和福斯高林等通过非受体依赖机制激活腺苷酸环化酶的试剂的作用。此外，ANF与血管紧张素II对大鼠主动脉腺苷酸环化酶的抑制反应表现出相加作用。这些研究首次证明ANF是多个系统中腺苷酸环化酶的抑制剂。
