人乳头瘤病毒16型重组DNA在猴肾细胞中作为自主复制的附加体维持存在。
Human papillomavirus type 16 recombinant DNA is maintained as an autonomously replicating episome in monkey kidney cells.
作者信息
Chesters P M, McCance D J
出版信息
J Gen Virol. 1985 Mar;66 ( Pt 3):615-20. doi: 10.1099/0022-1317-66-3-615.
PMID:2983004
Abstract
Human papillomavirus type 16 (HPV16) DNA cloned in the expression vector pSV2-neo has been shown to be maintained in transfected monkey kidney cells as an autonomously replicating episome at a level of 2 to 10 copies per cell. Integration of pSV2-neo/HPV16 DNA with the host genome occurred in transfected mouse fibroblasts. Neither type of cell appeared to be phenotypically transformed.
摘要
已证明克隆于表达载体pSV2-neo中的16型人乳头瘤病毒（HPV16）DNA在转染的猴肾细胞中作为自主复制的附加体得以维持，每个细胞中有2至10个拷贝。pSV2-neo/HPV16 DNA与宿主基因组的整合发生在转染的小鼠成纤维细胞中。这两种细胞均未表现出表型转化。
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