Sweet G H, Bryant S A, Tegtmeier G E, Beneke J S, Bayer W L
J Med Virol. 1985 Feb;15(2):137-48. doi: 10.1002/jmv.1890150206.
Early antigens (EA) of human cytomegalovirus extracted from cytosine arabinoside-blocked cells infected with 0.01-20 infectious units (IU)/cell were assayed with human serum by electroimmunodiffusion (EID). The number of detectable EA types increased from one to eight as the IU/cell was raised from 0.01 to 10. There was no increase in the number of EA with further increases in IU/cell, with prolonged culture, or when detergent was included in the extraction buffer. At least five of the eight EA gave reactions of identity with late-time antigens (LTA) extracted from unblocked cells at late times postinfection. In studies on a panel of sera from donors who were excreting virus and donors who were not, EID was as sensitive as conventional techniques (complement fixation and indirect hemagglutination for LTA, indirect immunofluorescence for EA) in detection of both types of antibodies from excretors but less sensitive in not detecting low levels of the antibodies in some of the sera from nonexcretors. No consistent relationships were observed between donor virological status and the numbers or types of antibodies to EA and LTA.
采用电免疫扩散法（EID），用人类血清检测从被0.01 - 20个感染单位（IU）/细胞感染的阿糖胞苷阻断细胞中提取的人巨细胞病毒早期抗原（EA）。随着IU/细胞从0.01增加到10，可检测到的EA类型数量从1种增加到8种。当IU/细胞进一步增加、培养时间延长或提取缓冲液中加入去污剂时，EA数量没有增加。8种EA中至少有5种与感染后期从未阻断细胞中提取的晚期抗原（LTA）产生相同反应。在对一组正在排出病毒的供体和未排出病毒的供体的血清进行的研究中，EID在检测排出者的两种抗体方面与传统技术（检测LTA的补体结合试验和间接血凝试验，检测EA的间接免疫荧光法）一样灵敏，但在检测一些未排出者血清中的低水平抗体方面不太灵敏。在供体的病毒学状态与针对EA和LTA的抗体数量或类型之间未观察到一致的关系。
