Middeldorp J M, Jongsma J, The T H
J Virol. 1985 Apr;54(1):240-4. doi: 10.1128/JVI.54.1.240-244.1985.
Using indirect immunofluorescence and a panel of human convalescent-phase sera, we identified cytomegalovirus (CMV) early and late membrane antigens (CMV-EMA and CMV-LMA, respectively) as separate entities on the surfaces of viable CMV-infected fibroblasts starting at 6 to 12 and 36 to 48 h postinoculation, respectively. For expression of CMV-EMA and CMV-LMA, infectious virus and active protein synthesis were required, whereas the expression of CMV-LMA, in addition, required viral DNA synthesis. Our data suggest that CMV-EMA and CMV-LMA form an individual set of CMV antigens that are different from intracellular CMV antigens and possibly (partly) different from the viral envelope.
利用间接免疫荧光法和一组人类恢复期血清，我们分别在接种后6至12小时和36至48小时开始，在存活的巨细胞病毒（CMV）感染的成纤维细胞表面，将巨细胞病毒早期和晚期膜抗原（分别为CMV-EMA和CMV-LMA）鉴定为不同的实体。CMV-EMA和CMV-LMA的表达需要感染性病毒和活跃的蛋白质合成，而CMV-LMA的表达此外还需要病毒DNA合成。我们的数据表明，CMV-EMA和CMV-LMA形成了一组独特的CMV抗原，它们不同于细胞内CMV抗原，并且可能（部分）不同于病毒包膜。
