Sweet G H, Tegtmeier G E, Bayer W L
J Gen Virol. 1979 Jun;43(3):707-12. doi: 10.1099/0022-1317-43-3-707.
The antigens of strain AD169 of human cytomegalovirus (CMV) were extracted by various methods and at different times following the appearance of cytopathic effects (c.p.e.) in infected fibroblasts. Assay with a pooled human serum in electroimmunodiffusion (EID) revealed that the most reactive preparations were obtained by shell-freeze (SF) extraction on the fourth day after 4+c.p.e. As many as 20 antigens could be detected in the original gels, most of which were stable upon storage at 4 degrees C for up to 4 weeks; of these, about 14 can be reproducibly seen on photographs. EID runs on day 4 SF preparations from high-passage CMV strains C87 and Davis and low passage recent isolates VD14, 1694 and 1723 resolved, respectively, 15, 15, 13, 11 and 11 antigens in the original gels (11, 9, 11,8 and 9 are visible in photographs). Strains 1694 and 1723 shared fewer antigens with one another and with high passage strains than were shared among the latter, whereas VD14 had relatively large numbers of antigens common to both low and high passage strains. At least six antigens were common to all strains.
采用多种方法，在感染的成纤维细胞出现细胞病变效应（c.p.e.）后的不同时间，提取人巨细胞病毒（CMV）AD169株的抗原。用电免疫扩散法（EID），以混合人血清进行检测，结果显示，在出现4+c.p.e.后的第4天，通过无壳冷冻（SF）提取获得的制剂反应性最强。在原始凝胶中可检测到多达20种抗原，其中大多数在4℃储存长达4周时保持稳定；其中，约14种抗原在照片上可重复看到。对高代CMV毒株C87和Davis以及低代近期分离株VD14、1694和1723在第4天的SF制剂进行EID分析，原始凝胶中分别分辨出15、15、13、11和11种抗原（照片上可见11、9、11、8和9种）。1694株和1723株彼此之间以及与高代毒株共有的抗原，比高代毒株之间共有的抗原少，而VD14具有相对大量的低代和高代毒株共有的抗原。所有毒株至少有6种抗原是共有的。
