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不同的膜锚定蛋白使塞姆利基森林病毒刺突亚基E2能够到达细胞表面。

Different membrane anchors allow the Semliki Forest virus spike subunit E2 to reach the cell surface.

作者信息

Riedel H

出版信息

J Virol. 1985 Apr;54(1):224-8. doi: 10.1128/JVI.54.1.224-228.1985.

DOI:10.1128/JVI.54.1.224-228.1985
PMID:2983116
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254781/
Abstract

The Semliki Forest virus spike subunit E2, a membrane-spanning protein, was transported to the plasma membrane in BHK cells after its carboxy terminus, including the intramembranous and cytoplasmic portions, was replaced by respective fragments of either the vesicular stomatitis virus glycoprotein or the fowl plague virus hemagglutinin. The hybrid proteins were constructed by cDNA fusion. Upon a transient expression they could be localized at the cell surface by immunofluorescence with specific antibodies directed against any of the protein fragments.

摘要

辛德毕斯病毒刺突亚基E2是一种跨膜蛋白，在其羧基末端（包括膜内和胞质部分）被水泡性口炎病毒糖蛋白或禽瘟病毒血凝素的相应片段取代后，在BHK细胞中被转运到质膜。通过cDNA融合构建杂种蛋白。瞬时表达后，用针对任何一种蛋白片段的特异性抗体进行免疫荧光检测，可将它们定位在细胞表面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b18/254781/999b82965916/jvirol00121-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b18/254781/51615566aa6f/jvirol00121-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b18/254781/999b82965916/jvirol00121-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b18/254781/51615566aa6f/jvirol00121-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b18/254781/999b82965916/jvirol00121-0237-a.jpg

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Different membrane anchors allow the Semliki Forest virus spike subunit E2 to reach the cell surface.不同的膜锚定蛋白使塞姆利基森林病毒刺突亚基E2能够到达细胞表面。
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Mutants of the membrane-binding region of Semliki Forest virus E2 protein. I. Cell surface transport and fusogenic activity.辛德毕斯病毒E2蛋白膜结合区域的突变体。I. 细胞表面转运与融合活性。

本文引用的文献

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Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.流感病毒血凝素膜糖蛋白在3埃分辨率下的结构
Nature. 1981 Jan 29;289(5796):366-73. doi: 10.1038/289366a0.
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Formation of the Semliki Forest virus membrane glycoprotein complexes in the infected cell.感染细胞中Semliki森林病毒膜糖蛋白复合物的形成。
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Antibodies to the Golgi complex and the rough endoplasmic reticulum.针对高尔基体和粗面内质网的抗体。
J Cell Biol. 1986 Mar;102(3):889-901. doi: 10.1083/jcb.102.3.889.
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Domains of virus glycoproteins.病毒糖蛋白的结构域。
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5
Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane.细胞和病毒整合膜蛋白的细胞质结构域在转运至质膜的过程中替代了水疱性口炎病毒糖蛋白的细胞质结构域。
J Cell Biol. 1986 Jun;102(6):2147-57. doi: 10.1083/jcb.102.6.2147.
J Cell Biol. 1982 Jan;92(1):92-107. doi: 10.1083/jcb.92.1.92.
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A monoclonal antibody against a 135-K Golgi membrane protein.一种针对135K高尔基体膜蛋白的单克隆抗体。
EMBO J. 1982;1(12):1621-8. doi: 10.1002/j.1460-2075.1982.tb01364.x.
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Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein.编码该蛋白质细胞内形式和分泌形式的流感血凝素基因的构建。
Nature. 1982 Dec 16;300(5893):598-603. doi: 10.1038/300598a0.
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Passage of viral membrane proteins through the Golgi complex.病毒膜蛋白通过高尔基体复合体的过程。
J Mol Biol. 1981 Nov 15;152(4):663-98. doi: 10.1016/0022-2836(81)90122-4.
7
Expression of the structural proteins of Semliki Forest virus from cloned cDNA microinjected into the nucleus of baby hamster kidney cells.将克隆的互补脱氧核糖核酸显微注射到幼仓鼠肾细胞核中后，塞姆利基森林病毒结构蛋白的表达
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4525-9. doi: 10.1073/pnas.79.15.4525.
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Cell surface expression of the influenza virus hemagglutinin requires the hydrophobic carboxy-terminal sequences.流感病毒血凝素的细胞表面表达需要疏水的羧基末端序列。
Cell. 1982 Sep;30(2):649-56. doi: 10.1016/0092-8674(82)90261-6.
9
Expression of Semliki Forest virus proteins from cloned complementary DNA. II. The membrane-spanning glycoprotein E2 is transported to the cell surface without its normal cytoplasmic domain.从克隆的互补DNA表达塞姆利基森林病毒蛋白。II. 跨膜糖蛋白E2在没有其正常胞质结构域的情况下被转运到细胞表面。
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10
Expression of Semliki Forest virus proteins from cloned complementary DNA. I. The fusion activity of the spike glycoprotein.从克隆的互补DNA表达Semliki森林病毒蛋白。I. 刺突糖蛋白的融合活性。
J Cell Biol. 1983 Sep;97(3):644-51. doi: 10.1083/jcb.97.3.644.