不同的膜锚定蛋白使塞姆利基森林病毒刺突亚基E2能够到达细胞表面。
Different membrane anchors allow the Semliki Forest virus spike subunit E2 to reach the cell surface.
作者信息
Riedel H
出版信息
J Virol. 1985 Apr;54(1):224-8. doi: 10.1128/JVI.54.1.224-228.1985.
Abstract
The Semliki Forest virus spike subunit E2, a membrane-spanning protein, was transported to the plasma membrane in BHK cells after its carboxy terminus, including the intramembranous and cytoplasmic portions, was replaced by respective fragments of either the vesicular stomatitis virus glycoprotein or the fowl plague virus hemagglutinin. The hybrid proteins were constructed by cDNA fusion. Upon a transient expression they could be localized at the cell surface by immunofluorescence with specific antibodies directed against any of the protein fragments.
摘要
辛德毕斯病毒刺突亚基E2是一种跨膜蛋白，在其羧基末端（包括膜内和胞质部分）被水泡性口炎病毒糖蛋白或禽瘟病毒血凝素的相应片段取代后，在BHK细胞中被转运到质膜。通过cDNA融合构建杂种蛋白。瞬时表达后，用针对任何一种蛋白片段的特异性抗体进行免疫荧光检测，可将它们定位在细胞表面。
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