Dotti C G, Kartenbeck J, Simons K
Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Germany.
Brain Res. 1993 Apr 30;610(1):141-7. doi: 10.1016/0006-8993(93)91227-j.
We have shown previously using immunofluorescence microscopy that upon infection of polarized hippocampal cells in culture with vesicular stomatitis virus (VSV) and fowl plague virus (FPV) the VSV glycoprotein is delivered to the plasma membrane of the dendrites and of the cell body whereas the FPV hemagglutinin is transported to the axonal surface (Cell, 62 (1990) 63-72). In this work electron microscopy of infected rat hippocampal neurons showed that VSV progeny budded from the plasma membrane of the dendrites and the cell body. The location of the budding virions corresponded to the distribution of the VSV glycoprotein which was detected over the somatodendritic plasma membrane by immunoelectron microscopy. In contrast, no FPV formation was seen in the infected neurons although the FPV hemagglutinin was localized to the axonal surface by immunoelectron microscopy. In Semliki Forest virus (SFV) infected hippocampal cells we observed that the viral glycoproteins were exclusively present in the dendrites and cell body but not in axons.
我们之前利用免疫荧光显微镜技术表明，在用水泡性口炎病毒（VSV）和禽瘟病毒（FPV）感染培养的极化海马细胞时，VSV糖蛋白被递送至树突和细胞体的质膜，而FPV血凝素则被运输至轴突表面（《细胞》，第62卷（1990年）63 - 72页）。在这项工作中，对受感染大鼠海马神经元的电子显微镜观察显示，VSV子代从树突和细胞体的质膜出芽。出芽病毒粒子的位置与通过免疫电子显微镜在体树突质膜上检测到的VSV糖蛋白的分布相对应。相比之下，尽管通过免疫电子显微镜观察到FPV血凝素定位于轴突表面，但在受感染的神经元中未观察到FPV的形成。在感染辛德毕斯病毒（SFV）的海马细胞中，我们观察到病毒糖蛋白仅存在于树突和细胞体中，而不存在于轴突中。
