Pass R F, Griffiths P D, August A M
J Infect Dis. 1983 Jan;147(1):40-6. doi: 10.1093/infdis/147.1.40.
The specific antibody response of 22 renal transplant patients with primary cytomegalo-virus (CMV) infection was compared to that of 21 patients with recurrent infection using seven different techniques to measure antibody. With primary infection seroconversion occurred between two and 12 weeks postoperatively, and geometric mean titers increased rapidly during this interval with each test except virus neutralization. In the group with recurrent CMV infection, geometric mean titers declined slightly initially and then increased rapidly to reach peak levels by 10 weeks. In both primary and recurrent infections, IgM antibody was detectable by radioimmunoassay and indirect immunofluorescence; the former procedure was clearly the more sensitive. After absorption to remove rheumatoid factor, 20 of the 22 patients with primary infection and eight of the 21 with recurrent infection had IgM antibody to CMV by radioimmunoassay which often persisted for over six months. The former group had significantly more viremia and symptomatic infections than the latter. Two critically ill patients failed to develop IgM or neutralizing antibody.
采用七种不同技术检测抗体，比较了22例原发性巨细胞病毒（CMV）感染的肾移植患者与21例复发性感染患者的特异性抗体反应。原发性感染时，血清转化发生在术后2至12周之间，在此期间，除病毒中和试验外，每种检测方法的几何平均滴度均迅速升高。在复发性CMV感染组中，几何平均滴度最初略有下降，然后迅速上升，至10周时达到峰值水平。在原发性和复发性感染中，均可通过放射免疫测定法和间接免疫荧光法检测到IgM抗体；前一种方法明显更敏感。去除类风湿因子后，22例原发性感染患者中有20例、21例复发性感染患者中有8例通过放射免疫测定法检测到CMV IgM抗体，该抗体通常持续超过6个月。原发性感染组的病毒血症和症状性感染明显多于复发性感染组。两名重症患者未能产生IgM或中和抗体。
