Hha I甲基化酶介导的DNA甲基化对Hae II、Aha II和Ban I核酸内切酶切割反应的影响。

The effects of DNA methylation by Hha I methylase on the cleavage reactions by Hae II, Aha II and Ban I endonucleases.

作者信息

Kang S C, Choi W S, Yoo O J

出版信息

Biochem Biophys Res Commun. 1987 May 29;145(1):482-7. doi: 10.1016/0006-291x(87)91346-5.

Abstract

The DNA methylated by Hha I methylase was resistant against cleavage of Hae II or Aha II endonuclease indicating that the methyl group of the C5 position of the inmost cytosine nucleotide interferes with the interaction between the enzyme and the hexameric recognition sequence. Considering that Hae II or Aha II methylase has not been isolated yet, the result explained above is a useful information for protecting a double stranded DNA from being cleaved by Hae II or Aha II endonuclease. In contrast to Hae II or Aha II endonuclease, Ban I endonuclease which also has Hha I sequence as its tetrameric core was able to cleave the same DNA normally. This result suggests that the C5 position of the inmost pyrimidine nucleotide is not an important contact point between Ban I endonuclease and its hexameric recognition sequence.

摘要

被Hha I甲基化酶甲基化的DNA对Hae II或Aha II核酸内切酶的切割具有抗性，这表明最内侧胞嘧啶核苷酸C5位的甲基会干扰该酶与六聚体识别序列之间的相互作用。鉴于尚未分离出Hae II或Aha II甲基化酶，上述结果对于保护双链DNA不被Hae II或Aha II核酸内切酶切割是有用的信息。与Hae II或Aha II核酸内切酶不同，同样以Hha I序列作为其四聚体核心的Ban I核酸内切酶能够正常切割相同的DNA。这一结果表明，最内侧嘧啶核苷酸的C5位不是Ban I核酸内切酶与其六聚体识别序列之间的重要接触点。