Azadmanesh Maryam, Noorbakhsh Mohammad Foad, Nazifi Saeed, Faraji Milad
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Oxid Med Cell Longev. 2025 Mar 6;2025:4421985. doi: 10.1155/omcl/4421985. eCollection 2025.
This study intends to investigate the green synthesis of silver (Ag) and gold (Au) nanoparticles (NPs) using extract and to evaluate the antioxidant and anti-inflammatory effects of the synthesized NPs and the extract. In this study, aqueous and hydroalcoholic extracts of were prepared, which were used for the biosynthesis of Ag and Au NPs. Dynamic light scattering (DLS), zeta potential analysis, transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, UV-Vis spectroscopy, and X-ray diffraction (XRD) methods were used to characterize the green NPs. The antioxidant effect of the NPs was estimated using in vitro methods, including reducing power (RP), ferric reducing/antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH). To evaluate the anti-inflammatory and antioxidant activity of extract and Ag and Au NPs, we used the carrageenan method. In our experiment, the extract and the synthesized NPs were administered orally to the mice 2 h before the carrageenan injection. The subsequent inhibition of inflammation and reduction of paw thickness were quantified. To evaluate their antioxidant effect, malondialdehyde (MDA), and total antioxidant capacity (TAC) levels were measured. Levels of pro-inflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor- (TNF-), were also quantified. In this study, the results indicate that the synthesized Ag and Au NPs have antioxidant and anti-inflammatory effects. The most promising results were observed in the groups that received the Ag NPs.
本研究旨在探究利用提取物进行银（Ag）和金（Au）纳米颗粒（NPs）的绿色合成，并评估合成的纳米颗粒和提取物的抗氧化及抗炎作用。在本研究中，制备了[提取物名称]的水提取物和水醇提取物，用于Ag和Au NPs的生物合成。采用动态光散射（DLS）、zeta电位分析、透射电子显微镜（TEM）、傅里叶变换红外（FT-IR）光谱、紫外可见光谱（UV-Vis）和X射线衍射（XRD）方法对绿色纳米颗粒进行表征。使用体外方法评估纳米颗粒的抗氧化作用，包括还原能力（RP）、铁还原/抗氧化能力（FRAP）和2,2-二苯基-1-苦基肼（DPPH）。为了评估[提取物名称]提取物以及Ag和Au NPs的抗炎和抗氧化活性，我们采用了角叉菜胶法。在我们的实验中，在注射角叉菜胶前2小时给小鼠口服提取物和合成的纳米颗粒。随后对炎症的抑制和爪厚度的减少进行量化。为了评估它们的抗氧化作用，测量了丙二醛（MDA）和总抗氧化能力（TAC）水平。还对促炎细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平进行了量化。在本研究中，结果表明合成的Ag和Au NPs具有抗氧化和抗炎作用。在接受Ag NPs的组中观察到了最有前景的结果。
