Schmidt A, Setoyama C, de Crombrugghe B
Nature. 1985;314(6008):286-9. doi: 10.1038/314286a0.
Oncogenic transformation of cells produces important changes in the biosynthetic pattern of certain cellular proteins. For example, the synthesis of type I collagen in transformed fibroblasts is severely reduced as a result of changes in transcription. Here we report the results of DNA-mediated transfection experiments using recombinant plasmids in which the promoter region of the alpha 2(I) collagen gene is fused to an easily recognizable marker gene, and cell lines expressing the marker gene are isolated. Our data show that the expression of the marker gene fused to the cloned alpha 2(I) collagen promoter is strongly inhibited by v-mos transformation, suggesting that a common mechanism inhibits both the transfected and endogeneous alpha 2(I) collagen promoters.
细胞的致癌转化会使某些细胞蛋白质的生物合成模式发生重要变化。例如，由于转录变化，转化的成纤维细胞中I型胶原蛋白的合成会严重减少。在此，我们报告了使用重组质粒进行DNA介导的转染实验的结果，其中α2（I）胶原蛋白基因的启动子区域与一个易于识别的标记基因融合，并分离出表达该标记基因的细胞系。我们的数据表明，与克隆的α2（I）胶原蛋白启动子融合的标记基因的表达受到v-mos转化的强烈抑制，这表明存在一种共同机制抑制转染的和内源性的α2（I）胶原蛋白启动子。
