长末端重复序列赋予骨髓增殖性肉瘤病毒造血转化特性。
Long terminal repeat sequences impart hematopoietic transformation properties to the myeloproliferative sarcoma virus.
作者信息
Stocking C, Kollek R, Bergholz U, Ostertag W
出版信息
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5746-50. doi: 10.1073/pnas.82.17.5746.
Abstract
The myeloproliferative sarcoma virus not only transforms fibroblasts but also causes extensive expansion of the hematopoietic stem cell compartment on infection of adult mice. Similar to the Moloney sarcoma virus, it carries the mos oncogene. Moloney sarcoma virus, however, does not induce myeloproliferation and leukemia in adult mice. The difference between the two viruses was explored by using their molecularly cloned genomes and the cellular mos oncogene to construct recombinant genomes. It was shown that the U3 region of the viral long terminal repeat (LTR) has a decisive function in determining the target cell specificity of the myeloproliferative sarcoma virus. Any mos gene, whether of cellular or viral origin, is sufficient in conjunction with the proper LTR to induce myeloproliferation. Our results indicate that the pathogenicity of acutely transforming viruses is determined not only by the oncogene but also by sequences in the viral LTR.
摘要
骨髓增殖性肉瘤病毒不仅能转化成纤维细胞，还会在感染成年小鼠时导致造血干细胞区室的广泛扩增。与莫洛尼肉瘤病毒相似，它携带mos癌基因。然而，莫洛尼肉瘤病毒不会在成年小鼠中诱导骨髓增殖和白血病。通过使用它们的分子克隆基因组和细胞mos癌基因构建重组基因组，对这两种病毒之间的差异进行了探索。结果表明，病毒长末端重复序列（LTR）的U3区域在决定骨髓增殖性肉瘤病毒的靶细胞特异性方面具有决定性作用。任何mos基因，无论是细胞来源还是病毒来源，与适当的LTR结合都足以诱导骨髓增殖。我们的结果表明，急性转化病毒的致病性不仅由癌基因决定，还由病毒LTR中的序列决定。
相似文献
1
Long terminal repeat sequences impart hematopoietic transformation properties to the myeloproliferative sarcoma virus.长末端重复序列赋予骨髓增殖性肉瘤病毒造血转化特性。
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5746-50. doi: 10.1073/pnas.82.17.5746.
2
Point mutations in the U3 region of the long terminal repeat of Moloney murine leukemia virus determine disease specificity of the myeloproliferative sarcoma virus.莫洛尼鼠白血病病毒长末端重复序列U3区域的点突变决定了骨髓增殖性肉瘤病毒的疾病特异性。
Virology. 1986 Aug;153(1):145-9. doi: 10.1016/0042-6822(86)90015-2.
3
The myeloproliferative sarcoma virus retains transforming functions after introduction of a dominant selectable marker gene.骨髓增殖性肉瘤病毒在引入显性选择标记基因后仍保留转化功能。
J Gen Virol. 1986 Jul;67 ( Pt 7):1361-71. doi: 10.1099/0022-1317-67-7-1361.
4
Long terminal repeat enhancement of v-mos transforming activity: identification of essential regions.v-mos转化活性的长末端重复序列增强：关键区域的鉴定
J Virol. 1983 Jun;46(3):726-36. doi: 10.1128/JVI.46.3.726-736.1983.
5
Rates of evolution of the retroviral oncogene of Moloney murine sarcoma virus and of its cellular homologues.莫洛尼氏鼠肉瘤病毒逆转录病毒癌基因及其细胞同源物的进化速率。
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4198-201. doi: 10.1073/pnas.82.12.4198.
6
Sequences in the long terminal repeats of the Moloney murine sarcoma virus-124 genome which control transforming gene function.莫洛尼氏鼠肉瘤病毒 - 124基因组的长末端重复序列中控制转化基因功能的序列。
Virology. 1984 Aug;137(1):32-40. doi: 10.1016/0042-6822(84)90005-9.
7
Study of the 78A1 isolate of Moloney murine sarcoma virus. I. Molecular cloning and characterization.莫洛尼鼠肉瘤病毒78A1分离株的研究。I. 分子克隆与特性分析。
J Gen Virol. 1985 Nov;66 ( Pt 11):2407-14. doi: 10.1099/0022-1317-66-11-2407.
8
Use of the mouse mammary tumor virus long terminal repeat to promote steroid-inducible expression of v-mos.利用小鼠乳腺肿瘤病毒长末端重复序列促进v-mos的类固醇诱导表达。
J Virol. 1984 Nov;52(2):420-30. doi: 10.1128/JVI.52.2.420-430.1984.
9
Temperature-sensitive viral RNA expression in Moloney murine sarcoma virus ts110-infected cells.莫洛尼氏鼠肉瘤病毒ts110感染细胞中温度敏感型病毒RNA的表达
J Virol. 1985 Feb;53(2):616-23. doi: 10.1128/JVI.53.2.616-623.1985.
10
Persistent expression of v-mos oncogene in transformed cells that revert to nonmalignancy after prolonged treatment with interferon.在用干扰素长时间处理后恢复为非恶性的转化细胞中v-mos癌基因的持续表达。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5764-8. doi: 10.1073/pnas.83.16.5764.
引用本文的文献
1
Development of safer gene delivery systems to minimize the risk of insertional mutagenesis-related malignancies: a critical issue for the field of gene therapy.开发更安全的基因递送系统以将插入诱变相关恶性肿瘤的风险降至最低：这是基因治疗领域的一个关键问题。
ISRN Oncol. 2012;2012:616310. doi: 10.5402/2012/616310. Epub 2012 Nov 22.
2
Production of replication-defective retrovirus by transient transfection of 293T cells.通过瞬时转染293T细胞产生复制缺陷型逆转录病毒。
J Vis Exp. 2007(10):550. doi: 10.3791/550. Epub 2007 Dec 4.
3
Insertional gene activation by lentiviral and gammaretroviral vectors.慢病毒载体和γ逆转录病毒载体介导的插入性基因激活
J Virol. 2009 Jan;83(1):283-94. doi: 10.1128/JVI.01865-08. Epub 2008 Oct 22.
4
In vivo analysis of retroviral enhancer mutations in hematopoietic cells: SP1/EGR1 and ETS/GATA motifs contribute to long terminal repeat specificity.造血细胞中逆转录病毒增强子突变的体内分析：SP1/EGR1和ETS/GATA基序对长末端重复序列特异性有贡献。
J Virol. 2002 Jan;76(1):303-12. doi: 10.1128/jvi.76.1.303-312.2002.
5
Insertional mutagenesis of preneoplastic astrocytes by Moloney murine leukemia virus.莫洛尼鼠白血病病毒对癌前星形胶质细胞的插入诱变
J Neurovirol. 2001 Apr;7(2):169-81. doi: 10.1080/13550280152058834.
6
Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells.经修饰的逆转录病毒载体在小鼠造血干细胞中持续、稳定的表达。
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10182-7. doi: 10.1073/pnas.95.17.10182.
7
Host cis-mediated extinction of a retrovirus permissive for expression in embryonal stem cells during differentiation.宿主顺式介导的逆转录病毒在分化过程中于胚胎干细胞中表达的许可性的消除。
J Virol. 1998 Jan;72(1):339-48. doi: 10.1128/JVI.72.1.339-348.1998.
8
Increased probability of expression from modified retroviral vectors in embryonal stem cells and embryonal carcinoma cells.在胚胎干细胞和胚胎癌细胞中，来自修饰逆转录病毒载体的表达概率增加。
J Virol. 1997 Dec;71(12):9466-74. doi: 10.1128/JVI.71.12.9466-9474.1997.
9
Amphotropic murine leukemia viruses induce spongiform encephalomyelopathy.双嗜性鼠白血病病毒可诱发海绵状脑脊髓病。
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5837-42. doi: 10.1073/pnas.94.11.5837.
10
Growth requirements and neoplastic transformation of two types of normal human breast epithelial cells derived from reduction mammoplasty.两种来自缩乳术的正常人类乳腺上皮细胞的生长需求及肿瘤转化
In Vitro Cell Dev Biol Anim. 1997 Apr;33(4):282-8. doi: 10.1007/s11626-997-0048-8.
本文引用的文献
1
The myeloproliferative sarcoma virus causes transformation or erythroid progenitor cells in vitro.骨髓增殖性肉瘤病毒在体外可导致红系祖细胞发生转化。
Mol Cell Biol. 1982 Feb;2(2):138-46. doi: 10.1128/mcb.2.2.138-146.1982.
2
Normal rat cell lines deficient in nuclear thymidine kinase.缺乏细胞核胸苷激酶的正常大鼠细胞系。
Virology. 1981 Aug;113(1):408-11. doi: 10.1016/0042-6822(81)90168-9.
3
Avian carcinoma virus MH2 contains a transformation-specific sequence, mht, and shares the myc sequence with MC29, CMII, and OK10 viruses.禽癌病毒MH2含有一个转化特异性序列mht，并与MC29、CMII和OK10病毒共享myc序列。
Proc Natl Acad Sci U S A. 1983 Nov;80(21):6566-70. doi: 10.1073/pnas.80.21.6566.
4
Action of temperature-sensitive mutants of myeloproliferative sarcoma virus suggests that fibroblast-transforming and hematopoietic transforming viral properties are related.骨髓增殖性肉瘤病毒温度敏感突变体的作用表明，成纤维细胞转化病毒特性和造血转化病毒特性是相关的。
J Virol. 1984 Jan;49(1):253-61. doi: 10.1128/JVI.49.1.253-261.1984.
5
The transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein.莫洛尼氏鼠肉瘤病毒的转化蛋白是一种可溶性细胞质蛋白。
Cell. 1983 May;33(1):161-72. doi: 10.1016/0092-8674(83)90345-8.
6
Comparison of myeloproliferative sarcoma virus with Moloney murine sarcoma virus variants by nucleotide sequencing and heteroduplex analysis.通过核苷酸测序和异源双链分析对骨髓增殖性肉瘤病毒与莫洛尼鼠肉瘤病毒变体进行比较。
J Virol. 1984 Jun;50(3):725-32. doi: 10.1128/JVI.50.3.725-732.1984.
7
Molecular cloning and characterization of a leukemia-inducing myeloproliferative sarcoma virus and two of its temperature-sensitive mutants.一种致白血病的骨髓增生性肉瘤病毒及其两个温度敏感突变体的分子克隆与特性分析。
J Virol. 1984 Jun;50(3):717-24. doi: 10.1128/JVI.50.3.717-724.1984.
8
Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat.通过长末端重复序列内的序列测定鼠逆转录病毒的致白血病性。
Nature. 1984;308(5958):467-70. doi: 10.1038/308467a0.
9
Molecular analysis of the envelope gene and long terminal repeat of Friend mink cell focus-inducing virus: implications for the functions of these sequences.弗氏貂细胞灶性诱导病毒包膜基因和长末端重复序列的分子分析：这些序列功能的启示
J Virol. 1984 Mar;49(3):828-40. doi: 10.1128/JVI.49.3.828-840.1984.
10
Avian oncovirus MH2: molecular cloning of proviral DNA and structural analysis of viral RNA and protein.禽肿瘤病毒MH2：前病毒DNA的分子克隆及病毒RNA与蛋白质的结构分析
J Virol. 1983 Oct;48(1):61-73. doi: 10.1128/JVI.48.1.61-73.1983.
Zotero 插件