Weisbart R H, Golde D W, Clark S C, Wong G G, Gasson J C
Nature. 1985;314(6009):361-3. doi: 10.1038/314361a0.
The polymorphonuclear leukocyte (PMN), or neutrophil, is the major host defence cell protecting the body against invasion by bacteria and fungi. Products of oxidative metabolism mediate PMN microbicidal and tumoricidal activity but the mechanisms by which these pathways become activated are not well understood. We have previously described a human granulocyte-macrophage colony-stimulating factor (GM-CSF) of relative molecular mass (Mr) 22,000 that also inhibits neutrophil motility (NIF-T activity). Because of its direct action on granulocytes, this lymphokine is a candidate for a neutrophil-activating factor. We have studied the effect of GM-CSF/NIF-T on superoxide anion generation in response to the bacterial chemo-attractant N-formylmethionyl-leucylphenylalanine (f-MLP), and report here that PMNs preincubated with either purified natural GM-CSF or biosynthetic (recombinant) GM-CSF showed increased (as much as fourfold) superoxide anion production in response to f-MLP. These results indicate that human GM-CSF is a neutrophil-activating factor.
多形核白细胞（PMN），即中性粒细胞，是保护机体抵御细菌和真菌入侵的主要宿主防御细胞。氧化代谢产物介导PMN的杀菌和杀肿瘤活性，但其激活这些途径的机制尚不清楚。我们之前描述了一种相对分子质量（Mr）为22,000的人粒细胞-巨噬细胞集落刺激因子（GM-CSF），它也抑制中性粒细胞运动（NIF-T活性）。由于其对粒细胞的直接作用，这种淋巴因子是中性粒细胞激活因子的候选者。我们研究了GM-CSF/NIF-T对响应细菌趋化因子N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（f-MLP）产生超氧阴离子的影响，在此报告，用纯化的天然GM-CSF或生物合成（重组）GM-CSF预孵育的PMN对f-MLP的超氧阴离子产生增加（高达四倍）。这些结果表明人GM-CSF是一种中性粒细胞激活因子。
