Apellániz Delmira, Pereira-Prado Vanesa, Sicco Estefania, Vigil-Bastitta Gabriela, González-González Rogelio, Mosqueda-Taylor Adalberto, Molina-Frechero Nelly, Hernandez Marcela, Sánchez-Romero Celeste, Bologna-Molina Ronell
1 Universidad de la República, Montevideo, Uruguay.
2 Universidad Juarez del Estado de Durango, Durango, México.
Int J Surg Pathol. 2018 Dec;26(8):714-720. doi: 10.1177/1066896918778341. Epub 2018 May 30.
Solid/conventional ameloblastoma (AM) and unicystic ameloblastoma (UAM) are the most frequent benign epithelial odontogenic tumors located in the maxillary region, and their treatment usually consists of extensive surgical resection. Therefore, it is relevant to study molecular markers to better understand the biological behavior of these tumors. The aim of this study was to describe and compare the expression of proteins related to cellular proliferation: Ki-67 and MCM4-6 complex.
An immunohistochemistry technique was performed, with antibodies against Ki-67, MCM4, MCM5, and MCM6, in 10 AM and 10 UAM tumors. The results were quantified using label index and analyzed statistically.
AM and UAM had greater expression of MCM6, followed by MCM5, MCM4, and Ki-67 ( P < .05). Immunoexpression of Ki-67 and MCM5 was exclusively nuclear, whereas the expression of MCM4 and MCM6 was nuclear and cytoplasmic.
The results suggest that MCM5 is a trustable cell proliferation marker with higher sensitivity compared with Ki-67 and may be useful to predict the biological behavior of AM and UAM. Despite this, further studies are necessary, including a correlation with clinical parameters to confirm these findings.
实性/传统型成釉细胞瘤（AM）和单囊性成釉细胞瘤（UAM）是位于上颌区域最常见的良性上皮性牙源性肿瘤，其治疗通常包括广泛的手术切除。因此，研究分子标志物以更好地了解这些肿瘤的生物学行为具有重要意义。本研究的目的是描述和比较与细胞增殖相关的蛋白质：Ki-67和MCM4-6复合物的表达。
采用免疫组织化学技术，用抗Ki-67、MCM4、MCM5和MCM6的抗体对10例AM肿瘤和10例UAM肿瘤进行检测。使用标记指数对结果进行定量并进行统计学分析。
AM和UAM中MCM6的表达更高，其次是MCM5、MCM4和Ki-67（P <.05）。Ki-67和MCM5的免疫表达仅位于细胞核，而MCM4和MCM6的表达位于细胞核和细胞质。
结果表明，与Ki-67相比，MCM5是一种更可靠、敏感性更高的细胞增殖标志物，可能有助于预测AM和UAM的生物学行为。尽管如此，仍需要进一步研究，包括与临床参数的相关性研究以证实这些发现。
