Department of Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
Head Neck Pathol. 2024 Oct 15;18(1):100. doi: 10.1007/s12105-024-01705-7.
To characterize the immunohistochemical expression of MDM2, Bcl-2, SATB2 and Ki-67 in histological variants of unicystic ameloblastoma (UA).
Following the ethical approval, forty (40) patients with unicystic ameloblastoma were retrieved from the archives and subjected to immunohistochemistry (IHC). Sociodemographic and clinical data were also retrieved. The results were entered into a Microsoft Excel spreadsheet and analyzed using SPSS software.
Human tooth germs, which served as the control, showed moderate expression of Bcl-2 and MDM2 with slight proliferative activity in ameloblasts and moderate expression of SATB2 in ectomesenchyme and odontoblasts. Luminal UA (Type 1) showed low Ki-67 index and negative to mild Bcl-2 and MDM2 expression, whilst Type 1.2 (luminal and intraluminal), Type 1.2.3 (luminal, intraluminal and mural), and Type 1.3 (luminal and mural), including the recurrent cases, showed moderate to intense expression with high mean Ki-67 index. The difference between the study groups was statistically significant (p value < 0.001). No expression of SATB2 was noted in any histological variant of UA. Furthermore, no significant differences were noted in age, gender, site and location between the groups.
In contrast to luminal variant of UA, mural±intraluminal variants and recurrent cases demonstrate higher expression of Bcl-2 and MDM2 with higher mean Ki-67 index. It may thus be prudent to provide aggressive treatment for cases, not just with mural follicles but also for the patients with intraluminal plexiform proliferation, to prevent recurrence and improve patient outcomes.
研究组织学变异型单囊型成釉细胞瘤（UA）中 MDM2、Bcl-2、SATB2 和 Ki-67 的免疫组织化学表达特征。
经伦理批准后，从档案中检索了 40 例单囊型成釉细胞瘤患者，并进行了免疫组织化学（IHC）检测。还检索了社会人口统计学和临床数据。结果输入 Microsoft Excel 电子表格，并使用 SPSS 软件进行分析。
人类牙胚作为对照，成釉细胞呈中度 Bcl-2 和 MDM2 表达，增殖活性轻微，外间充质和成牙本质细胞呈中度 SATB2 表达。管腔型 UA（1 型）Ki-67 指数低，Bcl-2 和 MDM2 表达阴性或轻度，而 1.2 型（管腔和管内）、1.2.3 型（管腔、管内和壁内）和 1.3 型（管腔和壁内），包括复发性病例，表现为中度至强烈表达，平均 Ki-67 指数较高。研究组之间的差异具有统计学意义（p 值<0.001）。UA 的任何组织学变异型均未见 SATB2 表达。此外，各组之间在年龄、性别、部位和位置方面无显著差异。
与管腔型 UA 相比，壁内±管内变异型和复发性病例表现出更高的 Bcl-2 和 MDM2 表达，以及更高的平均 Ki-67 指数。因此，不仅对壁内滤泡，而且对管内丛状增生的病例，给予积极的治疗可能是谨慎的，以防止复发，改善患者预后。
