Tamburus N Y, Verlengia R, Kunz V C, César M C, Silva E
Núcleo de Pesquisa em Exercício Físico, Departamento de Fisioterapia, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
Laboratório de Performance Humana, Faculdade de Ciências da Saúde, Universidade Metodista de Piracicaba, Piracicaba, SP, Brasil.
Braz J Med Biol Res. 2018;51(8):e6944. doi: 10.1590/1414-431x20186944. Epub 2018 May 28.
Physical training has been strongly recommended as a non-pharmacological treatment for coronary artery disease (CAD). Genetic polymorphisms have been studied to understand the biological variability in response to exercise among individuals. This study aimed to verify the possible influence of apolipoprotein B (ApoB: rs1042031 and rs693) and angiotensin-converting enzyme (ACE-ID: rs1799752) genotypes on the lipid profile and functional aerobic capacity, respectively, after an aerobic interval training (AIT) program in patients with CAD and/or cardiovascular risk factors. Sixty-six men were randomized and assigned to trained group (n=32) or control group (n=34). Cardiopulmonary exercise test was performed to determine the ventilatory anaerobic threshold (VAT) from cardiorespiratory variables. The AIT program, at an intensity equivalent to %VAT (70-110%), was conducted three times a week for 16 weeks. ApoB gene polymorphisms (-12669C>T (rs1042031) and -7673G>A (rs693)) were identified by real-time polymerase chain reaction (PCR). I/D polymorphism in the ACE gene (rs1799752) was identified through PCR and fragment size analysis. After 16 weeks, low-density lipoprotein (LDL) levels increased in the trained and control groups with the GA+AA genotype (-7673G>A) of the ApoB gene. Trained groups with ACE-II and ACE-ID genotypes presented an increase in oxygen consumption (VO2VAT) and power output after the AIT program. The presence of the ACE I-allele was associated with increased aerobic functional capacity after the AIT program. Increased LDL levels were observed over time in patients with the -7673G>A polymorphism of the ApoB gene. Trial Registration Information: ClinicalTrials.gov: NCT02313831.
体育锻炼已被强烈推荐作为冠状动脉疾病（CAD）的一种非药物治疗方法。人们对基因多态性进行了研究，以了解个体对运动反应的生物学变异性。本研究旨在验证载脂蛋白B（ApoB：rs1042031和rs693）和血管紧张素转换酶（ACE-ID：rs1799752）基因型分别对CAD和/或心血管危险因素患者进行有氧间歇训练（AIT）计划后的血脂谱和功能性有氧能力的可能影响。66名男性被随机分为训练组（n = 32）或对照组（n = 34）。进行心肺运动试验以根据心肺变量确定通气无氧阈（VAT）。AIT计划以相当于%VAT（70 - 110%）的强度，每周进行三次，共16周。通过实时聚合酶链反应（PCR）鉴定ApoB基因多态性（-12669C>T（rs1042031）和-7673G>A（rs693））。通过PCR和片段大小分析鉴定ACE基因（rs1799752）中的I/D多态性。16周后，ApoB基因GA + AA基因型（-7673G>A）的训练组和对照组中低密度脂蛋白（LDL）水平升高。具有ACE-II和ACE-ID基因型的训练组在AIT计划后氧耗量（VO2VAT）和功率输出增加。AIT计划后，ACE I等位基因的存在与有氧功能能力增加有关。ApoB基因-7673G>A多态性患者随时间观察到LDL水平升高。试验注册信息：ClinicalTrials.gov：NCT02313831。
