Verlengia Rozangela, Rebelo Ana C, Crisp Alex H, Kunz Vandeni C, Dos Santos Carneiro Cordeiro Marco A, Hirata Mario H, Crespo Hirata Rosario D, Silva Ester
College of Health Sciences, Methodist University of Piracicaba, Piracicaba, Brazil.
Department of Morphology, Federal University of Goias, Goiania, Brazil.
Asian J Sports Med. 2014 Sep;5(3):e22768. doi: 10.5812/asjsm.22768. Epub 2014 Aug 28.
Polymorphisms at the angiotensin-converting enzyme gene (ACE), such as the indel [rs1799752] variant in intron 16, have been shown to be associated with aerobic performance of athletes and non-athletes. However, the relationship between ACE indel polymorphism and cardiorespiratory fitness has not been always demonstrated.
The relationship between ACE indel polymorphism and cardiorespiratory fitness was investigated in a sample of young Caucasian Brazilian women.
This study investigated 117 healthy women (aged 18 to 30 years) who were grouped as physically active (n = 59) or sedentary (n = 58). All subjects performed an incremental exercise test (ramp protocol) on a cycle-ergometer with 20-25 W/min increments. Blood samples were obtained for DNA extraction and to analyze metabolic and hormonal profiles. ACE indel polymorphism was determined by polymerase chain reaction (PCR) and fragment size analysis.
The physically active group had higher values of peak oxygen uptake (VO2 peak), carbon dioxide output (VCO2), ventilation (VE) and power output than the sedentary group (P < 0.05) at the peak of the exercise test. However, heart rate (HR), systolic blood pressure (SBP) and diastolic blood pressure (DBP) did not differ between groups. There was no relationship between ACE indel polymorphism and cardiorespiratory variables during the test in both the physically active and sedentary groups, even when the dominant (DD vs. D1 + 2) and recessive (2 vs. DI + DD) models of inheritance were tested.
These results do not support the concept that the genetic variation at the ACE locus contributes to the cardiorespiratory responses at the peak of exercise test in physically active or sedentary healthy women. This indicates that other factors might mediate these responses, including the physical training level of the women.
血管紧张素转换酶基因（ACE）的多态性，如第16内含子中的插入/缺失[rs1799752]变异，已被证明与运动员和非运动员的有氧运动能力有关。然而，ACE插入/缺失多态性与心肺适能之间的关系并不总是得到证实。
在一组年轻的巴西白人女性样本中研究ACE插入/缺失多态性与心肺适能之间的关系。
本研究调查了117名健康女性（年龄18至30岁），她们被分为体育活动组（n = 59）或久坐组（n = 58）。所有受试者在功率自行车上进行递增运动测试（斜坡方案），功率以每分钟20 - 25瓦递增。采集血样用于DNA提取以及分析代谢和激素谱。通过聚合酶链反应（PCR）和片段大小分析确定ACE插入/缺失多态性。
在运动测试峰值时，体育活动组的峰值摄氧量（VO2峰值）、二氧化碳排出量（VCO2）、通气量（VE）和功率输出值均高于久坐组（P < 0.05）。然而，两组之间的心率（HR）、收缩压（SBP）和舒张压（DBP）没有差异。在体育活动组和久坐组的测试过程中，ACE插入/缺失多态性与心肺变量之间均无关联，即使对显性（DD与D1 + 2）和隐性（2与DI + DD）遗传模式进行测试时也是如此。
这些结果不支持ACE基因座的遗传变异有助于体育活动或久坐的健康女性在运动测试峰值时的心肺反应这一概念。这表明其他因素可能介导这些反应，包括女性的体育锻炼水平。
