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v-sis癌基因产物分泌形式的血小板衍生生长因子激动剂活性。

Platelet-derived growth factor agonist activity of a secreted form of the v-sis oncogene product.

作者信息

Johnsson A, Betsholtz C, von der Helm K, Heldin C H, Westermark B

出版信息

Proc Natl Acad Sci U S A. 1985 Mar;82(6):1721-5. doi: 10.1073/pnas.82.6.1721.

DOI:10.1073/pnas.82.6.1721
PMID:2984666
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397344/
Abstract

We have compared the functional properties of a growth factor partially purified from medium conditioned by simian sarcoma virus-transformed cells with those of platelet-derived growth factor (PDGF). The factor mimicked the effects induced by PDGF: it bound to and activated human fibroblast PDGF receptors and stimulated DNA synthesis. These activities were specifically inhibited by PDGF antibodies and thus elicited by a factor(s) immunologically related to PDGF. The factor behaved as a secretory protein, since about 95% of the receptor-binding activity was found in the medium after a 48-hr serum-free incubation. Structural characterization of the PDGF-like activity revealed a Mr 24,000 intracellular protein and two polypeptides of Mr 13,000 and 11,500 released into the medium. The Mr 13,000 component bound to human fibroblasts; this binding was competitively inhibited by PDGF. The data support the possibility that oncogene products may elicit transforming activity by interacting with the normal cellular mitogenic pathway.

摘要

我们已将从猿猴肉瘤病毒转化细胞条件培养基中部分纯化得到的生长因子的功能特性与血小板衍生生长因子（PDGF）的功能特性进行了比较。该因子模拟了PDGF诱导的效应：它能结合并激活人成纤维细胞PDGF受体，并刺激DNA合成。这些活性被PDGF抗体特异性抑制，因此是由与PDGF免疫相关的一种或多种因子引发的。该因子表现为一种分泌蛋白，因为在无血清孵育48小时后，约95%的受体结合活性存在于培养基中。对PDGF样活性的结构表征显示，有一种分子量为24,000的细胞内蛋白以及两种分子量分别为13,000和11,500的多肽释放到培养基中。分子量为13,000的成分能与人成纤维细胞结合；这种结合被PDGF竞争性抑制。这些数据支持了癌基因产物可能通过与正常细胞有丝分裂途径相互作用而引发转化活性的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/9c72f894d74d/pnas00346-0160-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/832c2091423e/pnas00346-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/3aea04b18b91/pnas00346-0159-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/ec870312b0fb/pnas00346-0159-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/e5a13619a953/pnas00346-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/19150eb51cb3/pnas00346-0160-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/dd2b904b2a36/pnas00346-0160-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/9c72f894d74d/pnas00346-0160-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/832c2091423e/pnas00346-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/3aea04b18b91/pnas00346-0159-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/ec870312b0fb/pnas00346-0159-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/e5a13619a953/pnas00346-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/19150eb51cb3/pnas00346-0160-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/dd2b904b2a36/pnas00346-0160-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/017a/397344/9c72f894d74d/pnas00346-0160-d.jpg

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本文引用的文献

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Platelet-derived growth factor: identification of constituent polypeptide chains.血小板衍生生长因子：组成多肽链的鉴定
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The phenotypic characteristics of simian sarcoma virus-transformed human fibroblasts suggest that the v-sis gene product acts solely as a PDGF receptor agonist in cell transformation.猿猴肉瘤病毒转化的人成纤维细胞的表型特征表明，v-sis基因产物在细胞转化中仅作为血小板衍生生长因子（PDGF）受体激动剂发挥作用。
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Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.从小鼠骨髓瘤肿瘤总RNA中纯化小鼠免疫球蛋白重链信使RNA。
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Transforming potential of human c-sis nucleotide sequences encoding platelet-derived growth factor.编码血小板衍生生长因子的人c-sis核苷酸序列的转化潜力
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