Leal F, Williams L T, Robbins K C, Aaronson S A
Science. 1985 Oct 18;230(4723):327-30. doi: 10.1126/science.2996133.
A scheme for partial purification of biologically active v-sis-coded protein from cells transformed with simian sarcoma virus (SSV) has made possible a functional comparison of the transforming protein with platelet-derived growth factor (PDGF). The SSV-transforming gene product is capable of specifically binding PDGF receptors, stimulating tyrosine phosphorylation of PDGF receptors, and inducing DNA synthesis in quiescent fibroblasts. Each of these activities was specifically inhibited by antibodies to different regions of the v-sis gene product. Moreover, viral infection of a variety of cell types revealed a strict correlation between those cells possessing PDGF receptors and those susceptible to transformation by SSV. These findings provide evidence that SSV-transforming activity is mediated by the interaction of a virus-coded mitogen with PDGF receptors.
一种从感染猿猴肉瘤病毒（SSV）的细胞中部分纯化具有生物活性的v-sis编码蛋白的方案，使得对该转化蛋白与血小板衍生生长因子（PDGF）进行功能比较成为可能。SSV转化基因产物能够特异性结合PDGF受体，刺激PDGF受体的酪氨酸磷酸化，并诱导静止成纤维细胞中的DNA合成。针对v-sis基因产物不同区域的抗体可特异性抑制上述每一项活性。此外，对多种细胞类型的病毒感染研究表明，拥有PDGF受体的细胞与那些易被SSV转化的细胞之间存在严格的相关性。这些发现提供了证据，表明SSV的转化活性是由病毒编码的有丝分裂原与PDGF受体的相互作用介导的。
