Aghakhani Chegeni Sara, Rahimzadeh Mahsa, Montazerghaem Hossein, Khayatian Mahmood, Dasturian Farzaneh, Naderi Nadereh
Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Food Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Biochem Genet. 2018 Dec;56(6):627-638. doi: 10.1007/s10528-018-9865-6. Epub 2018 May 30.
Cardiopulmonary bypass-associated acute kidney injury (CPB-AKI) is a well-recognized complication which is clearly linked to increased morbidity and mortality. Due to important role of inflammation in CPB-AKI pathogenesis, we explored the association between polymorphisms in STAT3, an inflammation-associated transcription factor, and the risk of CPB-AKI. In this study, STAT3 rs1053004 and rs744166 polymorphisms were analyzed in 129 patients undergoing coronary artery bypass grafting in Jorjani heart center, Bandar Abbas, Iran. The genotypes were determined using sequence-specific primers (PCR-SSP). Sixty-three patients met the criteria for AKI after cardiac surgery (AKI group). The remaining 66 patients did not develop AKI (non-AKI group). Rs1053004 GG genotype was significantly associated with a decreased risk (OR 0.4, 95% CI 0.17-0.9, P = 0.03) of CPB-AKI. Subgroup analyses revealed that GG genotype has also a protective effect in older patients (Age ≥ 60) (OR 0.19, 95% CI 0.04-0.8, P = 0.01). However, rs744166 did not show any difference between AKI and non-AKI groups. The result of our study for the first time provides evidence that rs1053004 polymorphism is significantly associated with a decreased risk of CPB-AKI in Iranian population, especially in older subjects.
体外循环相关急性肾损伤（CPB-AKI）是一种公认的并发症，与发病率和死亡率的增加明显相关。由于炎症在CPB-AKI发病机制中起重要作用，我们探讨了炎症相关转录因子STAT3的多态性与CPB-AKI风险之间的关联。在本研究中，对伊朗阿巴斯港乔尔贾尼心脏中心129例行冠状动脉搭桥术的患者进行了STAT3 rs1053004和rs744166多态性分析。使用序列特异性引物（PCR-SSP）确定基因型。63例患者心脏手术后符合急性肾损伤标准（急性肾损伤组）。其余66例患者未发生急性肾损伤（非急性肾损伤组）。Rs1053004 GG基因型与CPB-AKI风险降低显著相关（OR 0.4，95%CI 0.17-0.9，P = 0.03）。亚组分析显示，GG基因型在老年患者（年龄≥60岁）中也有保护作用（OR 0.19，95%CI 0.04-0.8，P = 0.01）。然而，rs744166在急性肾损伤组和非急性肾损伤组之间未显示任何差异。我们的研究结果首次提供了证据，表明rs1053004多态性与伊朗人群尤其是老年受试者CPB-AKI风险降低显著相关。
